Immobilization of lipase and its investigation

J. Temesvári, P. Biacs

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The enzymatic transformation of food industrial raw materials such as fats and oils, has been carried out with the use of immobilized lipases. This way the value of lower quality raw materials can be improved. First step of the bioconversion method is the immobilization of lipase. Lipase preparations of different origin (Rhizopus sp., porcine pancreas and Penicillium roqueforti) were immobilized on synthetic polymers (DUOLITE resin, PVC), on glass-based carrier (AP-685, ALD-610) and on aluminium oxide carrier. The conditions of the immobilization were studied in aqueous and hexaneous solvents, at various temperatures (20-60 °C) and pH-values (7.0-9.0). Immobilized lipase preparation with the greatest lipase activity was examined in column reactor, measuring the diminution of enzyme activity as a function of time. Following a 48 hours' reaction period, the relative activity decreased somewhat below 10%. Using immobilized lipase the bioconversional process can be regulated. From industrial waste fats and oils valuable raw material can be produced.

Original languageEnglish
Pages (from-to)277-290
Number of pages14
JournalActa Alimentaria
Volume25
Issue number3
Publication statusPublished - Sep 1996

Fingerprint

Lipase
Immobilization
raw materials
oils
Rhizopus
industrial wastes
aluminum oxide
lipids
biotransformation
Penicillium
pancreas
resins
glass
polymers
Fats
enzyme activity
Industrial Waste
Industrial Oils
swine
Aluminum Oxide

Keywords

  • DUOLITE ES 568, PVC, glass-based and aluminium oxide supports
  • Immobilization
  • Lipase, E.C.3.1.1.3

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Immobilization of lipase and its investigation. / Temesvári, J.; Biacs, P.

In: Acta Alimentaria, Vol. 25, No. 3, 09.1996, p. 277-290.

Research output: Contribution to journalArticle

Temesvári, J & Biacs, P 1996, 'Immobilization of lipase and its investigation', Acta Alimentaria, vol. 25, no. 3, pp. 277-290.
Temesvári, J. ; Biacs, P. / Immobilization of lipase and its investigation. In: Acta Alimentaria. 1996 ; Vol. 25, No. 3. pp. 277-290.
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