Immobilization engineering-How to design advanced sol-gel systems for biocatalysis?

Diána Weiser, Flóra Nagy, Gergely Bánóczi, Márk Oláh, Attila Farkas, A. Szilágyi, K. László, Ákos Gellért, G. Marosi, S. Kemény, L. Poppe

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

An immobilization engineering approach using bioinformatics and experimental design tools was applied to improve the sol-gel enzyme entrapment methodology. This strategy was used for the immobilization of lipase B from Candida antarctica (CaLB), a versatile enzyme widely used even on the industrial scale. The optimized entrapment of CaLB in sol-gel matrices is reported by the response-surface methodology enabling efficient process development. The immobilized CaLBs characterized by functional efficiency and enhanced recovery provided economical and green options for flow chemistry. Various ternary mixtures of sol-gel precursors allowed the creation of tailored entrapment matrices best suited for the enzyme and its targeted substrate. The sol-gel-entrapped forms of CaLB were excellent biocatalysts in the kinetic resolutions of secondary alcohols and secondary amines with aromatic or aliphatic substituents both in batch and continuous-flow biotransformations.

Original languageEnglish
Pages (from-to)3927-3937
Number of pages11
JournalGreen Chemistry
Volume19
Issue number16
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

immobilization
Sol-gels
gel
engineering
Enzymes
enzyme
Enhanced recovery
Biocatalysts
matrix
bioinformatics
Candida
Lipases
biotransformation
Bioinformatics
experimental design
Design of experiments
Amines
alcohol
Alcohols
substrate

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution

Cite this

Immobilization engineering-How to design advanced sol-gel systems for biocatalysis? / Weiser, Diána; Nagy, Flóra; Bánóczi, Gergely; Oláh, Márk; Farkas, Attila; Szilágyi, A.; László, K.; Gellért, Ákos; Marosi, G.; Kemény, S.; Poppe, L.

In: Green Chemistry, Vol. 19, No. 16, 01.01.2017, p. 3927-3937.

Research output: Contribution to journalArticle

Weiser, Diána ; Nagy, Flóra ; Bánóczi, Gergely ; Oláh, Márk ; Farkas, Attila ; Szilágyi, A. ; László, K. ; Gellért, Ákos ; Marosi, G. ; Kemény, S. ; Poppe, L. / Immobilization engineering-How to design advanced sol-gel systems for biocatalysis?. In: Green Chemistry. 2017 ; Vol. 19, No. 16. pp. 3927-3937.
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