Immobilization engineering-How to design advanced sol-gel systems for biocatalysis?

Diána Weiser, Flóra Nagy, Gergely Bánóczi, Márk Oláh, Attila Farkas, András Szilágyi, Krisztina László, Ákos Gellért, György Marosi, Sándor Kemény, László Poppe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

An immobilization engineering approach using bioinformatics and experimental design tools was applied to improve the sol-gel enzyme entrapment methodology. This strategy was used for the immobilization of lipase B from Candida antarctica (CaLB), a versatile enzyme widely used even on the industrial scale. The optimized entrapment of CaLB in sol-gel matrices is reported by the response-surface methodology enabling efficient process development. The immobilized CaLBs characterized by functional efficiency and enhanced recovery provided economical and green options for flow chemistry. Various ternary mixtures of sol-gel precursors allowed the creation of tailored entrapment matrices best suited for the enzyme and its targeted substrate. The sol-gel-entrapped forms of CaLB were excellent biocatalysts in the kinetic resolutions of secondary alcohols and secondary amines with aromatic or aliphatic substituents both in batch and continuous-flow biotransformations.

Original languageEnglish
Pages (from-to)3927-3937
Number of pages11
JournalGreen Chemistry
Volume19
Issue number16
DOIs
Publication statusPublished - Jan 1 2017

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution

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