Porcine pancreas lipase (triacylglycerol ester hydrolase, EC 184.108.40.206) was immobilized with the highest activity (2,187 U g-1 solid) on polyacrylamide beads possessing carboxylic functional groups activated by a water-soluble carbodiimide. The optimum pH for catalytic activity was pH 8.9. The apparent optimum temperature for the immobibized enzyme was about 7°C higher than that for the soluble enzyme. The immobilization stabilized the enzyme against heat and urea treatment. Cross-linking of the immobilized enzyme with glutaraldehyde or 3,5-difluoronitrobenzene improved the thermal stability. Application of the immobilized lipase for olive oil hydrolysis is also presented.
- Conformational stability
- Porcine pancreas lipase
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology