Immediate DNA ploidy analysis of gastrointestinal biopsies taken by endoscopy using a mechanical dissociation device

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9 Citations (Scopus)

Abstract

Background: Quantitative DNA analysis of fresh biopsy material can contribute to a more accurate diagnosis and prognosis. The authors aimed to develop and test a mechanical, nuclear preparation protocol for quantitative DNA analysis. Patients and Methods: Altogether 32 gastric (10 healthy, 17 gastritis, 7 adenocarcinoma) and 48 colon (21 healthy, 20 colitis ulcerosa, 7 adenocarcinoma) biopsy specimens were evaluated. The mechanical disruption was performed by Medimachine (DAKO, Denmark). The flow cytometry analysis was performed on a BD FACSStar flow cytometer. Results: DNA Aneuploidy was found in gastric samples only in tumours. The S-phase fraction of the normal cases was 5.9 ± 2.1%, 5.1% ± 1.2% in gastritis and 10.7 ± 1.6% in carcinomas. Seven out of 20 colitis ulcerosa and 4 out of 7 colon cancer samples were aneuploid. The S-phase fraction of normal colon cases was 5.7 ± 3.4%, in colitis 8.1 ± 4.2% and 15.1 ± 5.7% in carcinomas, respectively. Conclusion: Mechanical nuclear isolation is a useful method for flow cytometric DNA ploidy analysis of fresh biopsy samples.

Original languageEnglish
Pages (from-to)655-660
Number of pages6
JournalAnticancer research
Volume23
Issue number1 B
Publication statusPublished - Jan 1 2003

Keywords

  • DNA aneuploidy
  • Flow cytometry
  • Gastric biopsy
  • Mechanical nuclear preparation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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