Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry

Máte Szarka, Márton Szigeti, A. Guttman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Laser-induced fluorescence detection (LIF) is a powerful tool for the quantitative analysis of fluorescent molecules, widely used in glycan analysis with fluorophore labeled carbohydrates where each species has a common response factor. Electrospray ionization mass spectrometry (ESI-MS), on the other hand, while revealing important structural information about individual analytes, generally can have different response factors for different species. For simpler and improved quantitation with ESI-MS, laser-induced fluorescent images were collected at the Taylor cone of the electrospray interface, enabling simultaneous and robust optical (quantitative) and MS (qualitative) detection of fluorophore labeled sugars. The performance of this universally applicable, interface design independent imaging laser-induced fluorescent (iLIF) system was demonstrated using capillary electrophoresis (CE)-ESI-MS in the analysis of aminopyrene-trisulfonate labeled linear maltooligosaccharides and branched glycans from human immunoglobulin. The limit of detection (LOD) of the iLIF system was in this case 40 attomole. The intra- and interday quantitative (peak area) reproducibilities of the system (RSD) were 4.15% and 6.79%, respectively.

Original languageEnglish
Pages (from-to)7738-7743
Number of pages6
JournalAnalytical Chemistry
Volume91
Issue number12
DOIs
Publication statusPublished - Jun 18 2019

Fingerprint

Electrospray ionization
Mass spectrometry
Cones
Fluorescence
Imaging techniques
Fluorophores
Lasers
Polysaccharides
Capillary electrophoresis
Sugars
Immunoglobulins
Carbohydrates
Molecules
Chemical analysis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry. / Szarka, Máte; Szigeti, Márton; Guttman, A.

In: Analytical Chemistry, Vol. 91, No. 12, 18.06.2019, p. 7738-7743.

Research output: Contribution to journalArticle

@article{ffc6fd2fd74240798c17b62562da228b,
title = "Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry",
abstract = "Laser-induced fluorescence detection (LIF) is a powerful tool for the quantitative analysis of fluorescent molecules, widely used in glycan analysis with fluorophore labeled carbohydrates where each species has a common response factor. Electrospray ionization mass spectrometry (ESI-MS), on the other hand, while revealing important structural information about individual analytes, generally can have different response factors for different species. For simpler and improved quantitation with ESI-MS, laser-induced fluorescent images were collected at the Taylor cone of the electrospray interface, enabling simultaneous and robust optical (quantitative) and MS (qualitative) detection of fluorophore labeled sugars. The performance of this universally applicable, interface design independent imaging laser-induced fluorescent (iLIF) system was demonstrated using capillary electrophoresis (CE)-ESI-MS in the analysis of aminopyrene-trisulfonate labeled linear maltooligosaccharides and branched glycans from human immunoglobulin. The limit of detection (LOD) of the iLIF system was in this case 40 attomole. The intra- and interday quantitative (peak area) reproducibilities of the system (RSD) were 4.15{\%} and 6.79{\%}, respectively.",
author = "M{\'a}te Szarka and M{\'a}rton Szigeti and A. Guttman",
year = "2019",
month = "6",
day = "18",
doi = "10.1021/acs.analchem.9b01028",
language = "English",
volume = "91",
pages = "7738--7743",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Imaging Laser-Induced Fluorescence Detection at the Taylor Cone of Electrospray Ionization Mass Spectrometry

AU - Szarka, Máte

AU - Szigeti, Márton

AU - Guttman, A.

PY - 2019/6/18

Y1 - 2019/6/18

N2 - Laser-induced fluorescence detection (LIF) is a powerful tool for the quantitative analysis of fluorescent molecules, widely used in glycan analysis with fluorophore labeled carbohydrates where each species has a common response factor. Electrospray ionization mass spectrometry (ESI-MS), on the other hand, while revealing important structural information about individual analytes, generally can have different response factors for different species. For simpler and improved quantitation with ESI-MS, laser-induced fluorescent images were collected at the Taylor cone of the electrospray interface, enabling simultaneous and robust optical (quantitative) and MS (qualitative) detection of fluorophore labeled sugars. The performance of this universally applicable, interface design independent imaging laser-induced fluorescent (iLIF) system was demonstrated using capillary electrophoresis (CE)-ESI-MS in the analysis of aminopyrene-trisulfonate labeled linear maltooligosaccharides and branched glycans from human immunoglobulin. The limit of detection (LOD) of the iLIF system was in this case 40 attomole. The intra- and interday quantitative (peak area) reproducibilities of the system (RSD) were 4.15% and 6.79%, respectively.

AB - Laser-induced fluorescence detection (LIF) is a powerful tool for the quantitative analysis of fluorescent molecules, widely used in glycan analysis with fluorophore labeled carbohydrates where each species has a common response factor. Electrospray ionization mass spectrometry (ESI-MS), on the other hand, while revealing important structural information about individual analytes, generally can have different response factors for different species. For simpler and improved quantitation with ESI-MS, laser-induced fluorescent images were collected at the Taylor cone of the electrospray interface, enabling simultaneous and robust optical (quantitative) and MS (qualitative) detection of fluorophore labeled sugars. The performance of this universally applicable, interface design independent imaging laser-induced fluorescent (iLIF) system was demonstrated using capillary electrophoresis (CE)-ESI-MS in the analysis of aminopyrene-trisulfonate labeled linear maltooligosaccharides and branched glycans from human immunoglobulin. The limit of detection (LOD) of the iLIF system was in this case 40 attomole. The intra- and interday quantitative (peak area) reproducibilities of the system (RSD) were 4.15% and 6.79%, respectively.

UR - http://www.scopus.com/inward/record.url?scp=85067424498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067424498&partnerID=8YFLogxK

U2 - 10.1021/acs.analchem.9b01028

DO - 10.1021/acs.analchem.9b01028

M3 - Article

AN - SCOPUS:85067424498

VL - 91

SP - 7738

EP - 7743

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 12

ER -