Imaging Interorganelle Contacts and Local Calcium Dynamics at the ER-Mitochondrial Interface

György Csordás, P. Várnai, Tünde Golenár, Swati Roy, George Purkins, Timothy G. Schneider, Tamás Balla, György Hajnóczky

Research output: Contribution to journalArticle

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Abstract

The ER-mitochondrial junction provides a local calcium signaling domain that is critical for both matching energy production with demand and the control of apoptosis. Here, we visualize ER-mitochondrial contact sites and monitor the localized [Ca2+] changes ([Ca2+]ER-mt) using drug-inducible fluorescent interorganelle linkers. We show that all mitochondria have contacts with the ER, but plasma membrane (PM)-mitochondrial contacts are less frequent because of interleaving ER stacks in both RBL-2H3 and H9c2 cells. Single mitochondria display discrete patches of ER contacts and show heterogeneity in the ER-mitochondrial Ca2+ transfer. Pericam-tagged linkers revealed IP3-induced [Ca2+]ER-mt signals that exceeded 9 μM and endured buffering bulk cytoplasmic [Ca2+] increases. Altering linker length to modify the space available for the Ca2+ transfer machinery had a biphasic effect on [Ca2+]ER-mt signals. These studies provide direct evidence for the existence of high-Ca2+ microdomains between the ER and mitochondria and suggest an optimal gap width for efficient Ca2+ transfer.

Original languageEnglish
Pages (from-to)121-132
Number of pages12
JournalMolecular Cell
Volume39
Issue number1
DOIs
Publication statusPublished - Jul 2010

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Mitochondria
Calcium
Calcium Signaling
Cell Membrane
Apoptosis
Pharmaceutical Preparations

Keywords

  • Cellbio
  • Cellcycle
  • Signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Csordás, G., Várnai, P., Golenár, T., Roy, S., Purkins, G., Schneider, T. G., ... Hajnóczky, G. (2010). Imaging Interorganelle Contacts and Local Calcium Dynamics at the ER-Mitochondrial Interface. Molecular Cell, 39(1), 121-132. https://doi.org/10.1016/j.molcel.2010.06.029

Imaging Interorganelle Contacts and Local Calcium Dynamics at the ER-Mitochondrial Interface. / Csordás, György; Várnai, P.; Golenár, Tünde; Roy, Swati; Purkins, George; Schneider, Timothy G.; Balla, Tamás; Hajnóczky, György.

In: Molecular Cell, Vol. 39, No. 1, 07.2010, p. 121-132.

Research output: Contribution to journalArticle

Csordás, G, Várnai, P, Golenár, T, Roy, S, Purkins, G, Schneider, TG, Balla, T & Hajnóczky, G 2010, 'Imaging Interorganelle Contacts and Local Calcium Dynamics at the ER-Mitochondrial Interface', Molecular Cell, vol. 39, no. 1, pp. 121-132. https://doi.org/10.1016/j.molcel.2010.06.029
Csordás, György ; Várnai, P. ; Golenár, Tünde ; Roy, Swati ; Purkins, George ; Schneider, Timothy G. ; Balla, Tamás ; Hajnóczky, György. / Imaging Interorganelle Contacts and Local Calcium Dynamics at the ER-Mitochondrial Interface. In: Molecular Cell. 2010 ; Vol. 39, No. 1. pp. 121-132.
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