IgA autoantibodies in the pemphigoids and linear IgA bullous dermatosis

Barbara Horváth, Andrea Niedermeier, Eva Podstawa, Ralf Müller, Nicolas Hunzelmann, Sarolta Kárpáti, Michael Hertl

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28 Citations (Scopus)

Abstract

Background: Patients with bullous pemphigoid (BP), mucous membrane pemphigoid (MMP) and pemphigoid gestationis (PG) have IgG antibodies against BP180 and BP230, components of the hemidesmosomes. Patients with linear IgA bullous dermatosis (LABD) have IgA autoantibodies against a 97/120-kDa protein which is highly homologous to a shedded fragment of the BP180-ectodomain. Objectives: The aim of our study was to determine the incidence of IgA autoantibodies directed against BP180/BP230 in the pemphigoids and LABD and to determine the antigenic regions that are targeted by IgA autoantibodies. Methods: Utilizing baculovirus-expressed recombinant BP180 and BP230 proteins, we performed immunoblot analyses for IgA reactivity of sera from patients with BP (. n = 30), MMP (. n = 10), PG (. n = 6), LABD (. n = 6) and from control patients with non-related pruritic dermatoses (. n = 8). Results: IgA reactivity against BP180 and/or BP230 was detected in 19/30 of the BP, in 7/10 of the MMP, in 6/6 of the LABD and in 3/6 of the PG sera, respectively, but not in the control group. In all subgroups, the major antigenic site recognized by IgA antibodies was located within the NH2-terminus of the BP180-ectodomain, but only a minority of the sera showed also IgA reactivity against the BP180-NC16a-domain. IgA reactivity against the central domain of BP180 was more frequently seen than against its COOH-terminus. IgA against the COOH- and NH2-terminus of BP230, respectively, was detected in 6/30 of the BP, 1/10 of the MMP, 1/6 of the LABD and 0/8 control sera. Conclusion: IgA reactivity against BP180 and/or BP230 is a common finding in the pemphigoids.

Original languageEnglish
Pages (from-to)648-653
Number of pages6
JournalExperimental Dermatology
Volume19
Issue number7
DOIs
Publication statusPublished - Jul 1 2010

Keywords

  • Bullous pemphigoid
  • IgA antibodies
  • Linear IgA bullous dermatosis
  • Mucous membrane pemphigoid
  • Pemphigoid gestationis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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  • Cite this

    Horváth, B., Niedermeier, A., Podstawa, E., Müller, R., Hunzelmann, N., Kárpáti, S., & Hertl, M. (2010). IgA autoantibodies in the pemphigoids and linear IgA bullous dermatosis. Experimental Dermatology, 19(7), 648-653. https://doi.org/10.1111/j.1600-0625.2010.01080.x