Identifying novel genes involved in both deer physiological and human pathological osteoporosis

Adrienn Borsy, János Podani, Viktor Stéger, Bernadett Balla, Arnold Horváth, János P. Kósa, István Gyurján, Andrea Molnár, Zoltán Szabolcsi, László Szabó, Eéna Jakó, Zoltán Zomborszky, János Nagy, Szabolcs Semsey, Tibor Vellai, Péter Lakatos, László Orosz

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Osteoporosis attacks 10% of the population worldwide. Humans or even the model animals of the disease cannot recover from porous bone. Regeneration in skeletal elements is the unique feature of our newly investigated osteoporosis model, the red deer (Cervus elaphus) stag. Cyclic physiological osteoporosis is a consequence of the annual antler cycle. This phenomenon raises the possibility to identify genes involved in the regulation of bone mineral density on the basis of comparative genomics between deer and human. We compare gene expression activity of osteoporotic and regenerating rib bone samples versus autumn dwell control in red deer by microarray hybridization. Identified genes were tested on human femoral bone tissue from non-osteoporotic controls and patients affected with age-related osteoporosis. Expression data were evaluated by Principal Components Analysis and Canonical Variates Analysis. Separation of patients into a normal and an affected group based on ten formerly known osteoporosis reference genes was significantly improved by expanding the data with newly identified genes. These genes include IGSF4, FABP3, FABP4, FKBP2, TIMP2, TMSB4X, TRIB, and members of the Wnt signaling. This study supports that extensive comparative genomic analyses, here deer and human, provide a novel approach to identify new targets for human diagnostics and therapy.

Original languageEnglish
Pages (from-to)301-313
Number of pages13
JournalMolecular Genetics and Genomics
Volume281
Issue number3
DOIs
Publication statusPublished - Mar 1 2009

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Keywords

  • Bone metabolism
  • Gene expression pattern
  • Interspecific microarray
  • Real-time PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Borsy, A., Podani, J., Stéger, V., Balla, B., Horváth, A., Kósa, J. P., Gyurján, I., Molnár, A., Szabolcsi, Z., Szabó, L., Jakó, E., Zomborszky, Z., Nagy, J., Semsey, S., Vellai, T., Lakatos, P., & Orosz, L. (2009). Identifying novel genes involved in both deer physiological and human pathological osteoporosis. Molecular Genetics and Genomics, 281(3), 301-313. https://doi.org/10.1007/s00438-008-0413-7