A Helicobacter pylori kimutatásának DNS-alapú diagnosztikai módszerei

Translated title of the contribution: Identifying Helicobacter pylori with DNA-based assays

Ágnes Ruzsovics, B. Molnár, Z. Tulassay

Research output: Contribution to journalArticle

Abstract

The DNA-based assays have the potential to be a powerful diagnostic tool given its ability to specifically identify H. pylori DNA. Markers used include general H. pylori structures and pathogenetic factors like ureaseA, cagA, vacA, iceA. DNA or bacterial RNA for polymerase chain reaction (PCR) assays can be collected from gastric biopsy, gastric juice, stool, buccal specimens. PCR can yield quantitative and genotyping results with sensitivity and specificity that approaches 100%. A clear trend in the direction of the determination of quantitative H. pylori infection by real-time PCR can be observed. Fluorescent in situ hybridisation (FISH) and restriction fragment length polymorphism (RFLP) are suggested for routine antibiotic resistance determination. To identify the DNA structure of organism and its virulence factors may be feasible by using oligonucleotide micro-array specifically recognising and discriminating bacterial DNA and various virulence factors. DNA based H. pylori diagnosis yields higher sensitivity, however, specificity requires sophisticated labour environment and associated with higher costs.

Original languageHungarian
Pages (from-to)337-342
Number of pages6
JournalLege Artis Medicinae
Volume14
Issue number5
Publication statusPublished - May 2004

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Helicobacter pylori
DNA
Virulence Factors
Bacterial RNA
Sensitivity and Specificity
Bacterial DNA
Polymerase Chain Reaction
Gastric Juice
Cheek
Helicobacter Infections
DNA-Directed RNA Polymerases
Microbial Drug Resistance
Oligonucleotide Array Sequence Analysis
Fluorescence In Situ Hybridization
Restriction Fragment Length Polymorphisms
Real-Time Polymerase Chain Reaction
Stomach
Biopsy
Costs and Cost Analysis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A Helicobacter pylori kimutatásának DNS-alapú diagnosztikai módszerei. / Ruzsovics, Ágnes; Molnár, B.; Tulassay, Z.

In: Lege Artis Medicinae, Vol. 14, No. 5, 05.2004, p. 337-342.

Research output: Contribution to journalArticle

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