Identifying calpain substrates in intact S2 cells of Drosophila

Zoltan Bozoky, Anita Alexa, Julia Dancsok, Gergo Gogl, E. Klement, Katalin F. Medzihradszky, P. Friedrich

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Calpains are cysteine proteases involved in a number of physiological and pathological processes, yet our knowledge of substrates cleaved in vivo, in intact cells, is scarce. In this work we made an attempt to develop a technique for finding calpain substrates in intact Drosophila Schneider S2 cells. The procedure consists in comparative 2D gelelectrophoresis: three identical samples were treated in different ways: A (control, no addition), B, activated (Ca2+ and ionomycin added), C, inactivated (additions as in B + specific calpain inhibitor). 2D gel pattern were analyzed by densitometry. Spots showing density relation A > B

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume481
Issue number2
DOIs
Publication statusPublished - Jan 15 2009

Fingerprint

Calpain
Drosophila
Physiological Phenomena
Ionomycin
Cysteine Proteases
Densitometry
Pathologic Processes
Substrates
Gels
calpain inhibitors

Keywords

  • 2D-electrophoresis
  • Calpain substrate
  • Drosophila
  • In vivo detection
  • Schneider cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identifying calpain substrates in intact S2 cells of Drosophila. / Bozoky, Zoltan; Alexa, Anita; Dancsok, Julia; Gogl, Gergo; Klement, E.; Medzihradszky, Katalin F.; Friedrich, P.

In: Archives of Biochemistry and Biophysics, Vol. 481, No. 2, 15.01.2009, p. 219-225.

Research output: Contribution to journalArticle

Bozoky, Zoltan ; Alexa, Anita ; Dancsok, Julia ; Gogl, Gergo ; Klement, E. ; Medzihradszky, Katalin F. ; Friedrich, P. / Identifying calpain substrates in intact S2 cells of Drosophila. In: Archives of Biochemistry and Biophysics. 2009 ; Vol. 481, No. 2. pp. 219-225.
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