Identification of unknown isomers of fumonisin B5 mycotoxin in a fusarium verticillioides culture by high-performance liquid chromatography/electrospray ionization time-of-flight and ion trap mass spectrometry

T. Bartók, László Tölgyesi, Árpád Szécsi, J. Varga, M. Bartók, Ákos Mesterházy, Ernö Gyimes, Antal Véha

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Several new fumonisin B5 mycotoxin isomers (iso-FB5) present in a rice culture inoculated with Fusarium verticillioides were separated on a RP-HPLC column recommended for the separation of structural isomers, and subsequently detected by time-of-flight and ion trap mass spectrometry. The exact masses of the eluted compounds were determined by using time-of-flight mass spectrometry. The m/z values of the 20 characteristic product ions including the hydrocarbon backbone and the characteristic neutral mass losses from the molecular and product ions of the compounds obtained by ion trap mass spectrometry, unambiguously indicated of the unknown iso-FB 5 compounds. The total amount of FB5 toxin and its isomers was 0.9% of the quantity of the main compound (FB1 toxin), which is a noteworthy quantity considering their potential toxicity. Based on the semi-quantitative measurement data it can be assumed that, in addition to the FB5 of unknown structure, isomers designated 1, 2, and 20 can also be isolated among the iso-FB5 compounds.

Original languageEnglish
Pages (from-to)1549-1561
Number of pages13
JournalJournal of Liquid Chromatography and Related Technologies
Volume36
Issue number11
DOIs
Publication statusPublished - Apr 1 2013

Fingerprint

Fumonisins
Electrospray ionization
Mycotoxins
Fusarium
High performance liquid chromatography
Isomers
Mass spectrometry
Mass Spectrometry
High Pressure Liquid Chromatography
Ions
Hydrocarbons
Toxicity

Keywords

  • FB
  • fumonisin B isomers
  • Fusarium verticillioides
  • LC-MS
  • LC-MS/MS
  • mycotoxin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Biochemistry
  • Clinical Biochemistry

Cite this

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abstract = "Several new fumonisin B5 mycotoxin isomers (iso-FB5) present in a rice culture inoculated with Fusarium verticillioides were separated on a RP-HPLC column recommended for the separation of structural isomers, and subsequently detected by time-of-flight and ion trap mass spectrometry. The exact masses of the eluted compounds were determined by using time-of-flight mass spectrometry. The m/z values of the 20 characteristic product ions including the hydrocarbon backbone and the characteristic neutral mass losses from the molecular and product ions of the compounds obtained by ion trap mass spectrometry, unambiguously indicated of the unknown iso-FB 5 compounds. The total amount of FB5 toxin and its isomers was 0.9{\%} of the quantity of the main compound (FB1 toxin), which is a noteworthy quantity considering their potential toxicity. Based on the semi-quantitative measurement data it can be assumed that, in addition to the FB5 of unknown structure, isomers designated 1, 2, and 20 can also be isolated among the iso-FB5 compounds.",
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AU - Tölgyesi, László

AU - Szécsi, Árpád

AU - Varga, J.

AU - Bartók, M.

AU - Mesterházy, Ákos

AU - Gyimes, Ernö

AU - Véha, Antal

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