Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans

Josep V. Forment, M. Flipphi, Daniel Ramón, Luisa Ventura, Andrew P. MacCabe

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified. mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA. The expression of MstE and its subcellular distribution have been studied using a MstE-sGFP fusion protein. Concordant with data on mstE expression, MstE-sGFP is synthesized in the presence of repressing carbon sources, and fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane. Deletion of mstE has no morphological phenotype but results in the absence of low affinity glucose uptake kinetics, the latter being substituted by a high affinity system.

Original languageEnglish
Pages (from-to)8339-8346
Number of pages8
JournalJournal of Biological Chemistry
Volume281
Issue number13
DOIs
Publication statusPublished - Mar 31 2006

Fingerprint

Aspergillus nidulans
Gene encoding
Facilitative Glucose Transport Proteins
Aspergillus
Fungal Spores
Hyphae
Glucose
Carbon
Genes
Cell membranes
Germination
Fusion reactions
Fluorescence
Cell Membrane
Phenotype
Kinetics
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans. / Forment, Josep V.; Flipphi, M.; Ramón, Daniel; Ventura, Luisa; MacCabe, Andrew P.

In: Journal of Biological Chemistry, Vol. 281, No. 13, 31.03.2006, p. 8339-8346.

Research output: Contribution to journalArticle

Forment, Josep V. ; Flipphi, M. ; Ramón, Daniel ; Ventura, Luisa ; MacCabe, Andrew P. / Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 13. pp. 8339-8346.
@article{80d85cea518f441ba1c02e830407ce53,
title = "Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans",
abstract = "The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified. mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA. The expression of MstE and its subcellular distribution have been studied using a MstE-sGFP fusion protein. Concordant with data on mstE expression, MstE-sGFP is synthesized in the presence of repressing carbon sources, and fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane. Deletion of mstE has no morphological phenotype but results in the absence of low affinity glucose uptake kinetics, the latter being substituted by a high affinity system.",
author = "Forment, {Josep V.} and M. Flipphi and Daniel Ram{\'o}n and Luisa Ventura and MacCabe, {Andrew P.}",
year = "2006",
month = "3",
day = "31",
doi = "10.1074/jbc.M508198200",
language = "English",
volume = "281",
pages = "8339--8346",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "13",

}

TY - JOUR

T1 - Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans

AU - Forment, Josep V.

AU - Flipphi, M.

AU - Ramón, Daniel

AU - Ventura, Luisa

AU - MacCabe, Andrew P.

PY - 2006/3/31

Y1 - 2006/3/31

N2 - The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified. mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA. The expression of MstE and its subcellular distribution have been studied using a MstE-sGFP fusion protein. Concordant with data on mstE expression, MstE-sGFP is synthesized in the presence of repressing carbon sources, and fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane. Deletion of mstE has no morphological phenotype but results in the absence of low affinity glucose uptake kinetics, the latter being substituted by a high affinity system.

AB - The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified. mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA. The expression of MstE and its subcellular distribution have been studied using a MstE-sGFP fusion protein. Concordant with data on mstE expression, MstE-sGFP is synthesized in the presence of repressing carbon sources, and fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane. Deletion of mstE has no morphological phenotype but results in the absence of low affinity glucose uptake kinetics, the latter being substituted by a high affinity system.

UR - http://www.scopus.com/inward/record.url?scp=33646832389&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646832389&partnerID=8YFLogxK

U2 - 10.1074/jbc.M508198200

DO - 10.1074/jbc.M508198200

M3 - Article

C2 - 16418173

AN - SCOPUS:33646832389

VL - 281

SP - 8339

EP - 8346

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 13

ER -