Identification of the blue-sensitive cones in the mammalian retina by anti-visual pigment antibody

A. Szél, T. Diamantstein, P. Röhlich

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Monoclonal antibodies to visual pigments produced in our laboratory were applied to analyze the distribution of color-specific photoreceptor cells in the retina (photoreceptor mosaic). We demonstrated in two ways that the monoclonal antibody OS-2 specifically recognized the blue-sensitive cone cells in the mammalian retina. First, rabbit photoreceptors damaged selectively by intense blue light were recognized by OS-2 antibody. Second, OS-2-positive cones in the ground squirrel were those with thick inner segments, which is known to be characteristic of the blue-sensitive cones. In addition, the OS-2-positive cones in monkeys have a distribution and pattern characteristic of blue-sensitive cones in that species. In several other species (human, rabbit, cow and pig), the OS-2-positive cones represent an appropriate minority of the population of photoreceptor cells. The visual pigment recognized by the OS-2 antibody had a relative molecular weight of 36,000, as shown by immunoblotting of 3 mammalian species. All other cones were recognized by another monoclonal antibody, COS-1, which is regarded as specific to middle-to-long wavelength-sensitive photoreceptors.

Original languageEnglish
Pages (from-to)593-602
Number of pages10
JournalJournal of Comparative Neurology
Volume273
Issue number4
Publication statusPublished - 1988

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Retinal Pigments
Retina
Photoreceptor Cells
Monoclonal Antibodies
Antibodies
Rabbits
Sciuridae
Immunoblotting
Haplorhini
Swine
Color
Molecular Weight
Light
Population

ASJC Scopus subject areas

  • Neuroscience(all)

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Identification of the blue-sensitive cones in the mammalian retina by anti-visual pigment antibody. / Szél, A.; Diamantstein, T.; Röhlich, P.

In: Journal of Comparative Neurology, Vol. 273, No. 4, 1988, p. 593-602.

Research output: Contribution to journalArticle

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