Identification of the binding site between bovine serum albumin and ultrasmall SiC fluorescent biomarkers

Gabriella Dravecz, Tibor Z. Jánosi, Dávid Beke, Dániel Major, Gyula Károlyházy, János Erostyák, K. Kamarás, A. Gali

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ultrasmall silicon carbide nanoparticles (SiC USNPs) are very promising biomarkers for developing new applications in diagnostics, cell monitoring or drug delivery, even though their interaction with biological molecules such as different proteins has not yet been investigated in detail. In this study, the biological behaviour of SiC USNPs in a medium modeling a living organism was investigated in detail through the dependence of the fluorescence on interactions between bovine serum albumin (BSA) and SiC USNPs. The interaction shows transient nanoparticle-protein associations due to the restricted diffusion behaviour of the nanoparticles in the vicinity of a protein. The transient association manifests in a complex fluorescence quenching mechanism where the dynamic component was dominated by Förster resonance energy transfer. By studying SiC nanoparticles of different sizes, it can be concluded that the transient effect is an ultrasmall nanoparticle behaviour.

Original languageEnglish
Pages (from-to)13419-13429
Number of pages11
JournalPhysical Chemistry Chemical Physics
Volume20
Issue number19
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

biomarkers
Biomarkers
Bovine Serum Albumin
albumins
serums
Binding Sites
Nanoparticles
nanoparticles
proteins
Fluorescence
fluorescence
Proteins
interactions
Drug delivery
organisms
silicon carbides
Energy transfer
Quenching
delivery
drugs

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Cite this

Identification of the binding site between bovine serum albumin and ultrasmall SiC fluorescent biomarkers. / Dravecz, Gabriella; Jánosi, Tibor Z.; Beke, Dávid; Major, Dániel; Károlyházy, Gyula; Erostyák, János; Kamarás, K.; Gali, A.

In: Physical Chemistry Chemical Physics, Vol. 20, No. 19, 01.01.2018, p. 13419-13429.

Research output: Contribution to journalArticle

Dravecz, Gabriella ; Jánosi, Tibor Z. ; Beke, Dávid ; Major, Dániel ; Károlyházy, Gyula ; Erostyák, János ; Kamarás, K. ; Gali, A. / Identification of the binding site between bovine serum albumin and ultrasmall SiC fluorescent biomarkers. In: Physical Chemistry Chemical Physics. 2018 ; Vol. 20, No. 19. pp. 13419-13429.
@article{1f63af00bc3547379355f4ee0c72eeff,
title = "Identification of the binding site between bovine serum albumin and ultrasmall SiC fluorescent biomarkers",
abstract = "Ultrasmall silicon carbide nanoparticles (SiC USNPs) are very promising biomarkers for developing new applications in diagnostics, cell monitoring or drug delivery, even though their interaction with biological molecules such as different proteins has not yet been investigated in detail. In this study, the biological behaviour of SiC USNPs in a medium modeling a living organism was investigated in detail through the dependence of the fluorescence on interactions between bovine serum albumin (BSA) and SiC USNPs. The interaction shows transient nanoparticle-protein associations due to the restricted diffusion behaviour of the nanoparticles in the vicinity of a protein. The transient association manifests in a complex fluorescence quenching mechanism where the dynamic component was dominated by F{\"o}rster resonance energy transfer. By studying SiC nanoparticles of different sizes, it can be concluded that the transient effect is an ultrasmall nanoparticle behaviour.",
author = "Gabriella Dravecz and J{\'a}nosi, {Tibor Z.} and D{\'a}vid Beke and D{\'a}niel Major and Gyula K{\'a}rolyh{\'a}zy and J{\'a}nos Erosty{\'a}k and K. Kamar{\'a}s and A. Gali",
year = "2018",
month = "1",
day = "1",
doi = "10.1039/c8cp02144a",
language = "English",
volume = "20",
pages = "13419--13429",
journal = "Physical Chemistry Chemical Physics",
issn = "1463-9076",
publisher = "Royal Society of Chemistry",
number = "19",

}

TY - JOUR

T1 - Identification of the binding site between bovine serum albumin and ultrasmall SiC fluorescent biomarkers

AU - Dravecz, Gabriella

AU - Jánosi, Tibor Z.

AU - Beke, Dávid

AU - Major, Dániel

AU - Károlyházy, Gyula

AU - Erostyák, János

AU - Kamarás, K.

AU - Gali, A.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Ultrasmall silicon carbide nanoparticles (SiC USNPs) are very promising biomarkers for developing new applications in diagnostics, cell monitoring or drug delivery, even though their interaction with biological molecules such as different proteins has not yet been investigated in detail. In this study, the biological behaviour of SiC USNPs in a medium modeling a living organism was investigated in detail through the dependence of the fluorescence on interactions between bovine serum albumin (BSA) and SiC USNPs. The interaction shows transient nanoparticle-protein associations due to the restricted diffusion behaviour of the nanoparticles in the vicinity of a protein. The transient association manifests in a complex fluorescence quenching mechanism where the dynamic component was dominated by Förster resonance energy transfer. By studying SiC nanoparticles of different sizes, it can be concluded that the transient effect is an ultrasmall nanoparticle behaviour.

AB - Ultrasmall silicon carbide nanoparticles (SiC USNPs) are very promising biomarkers for developing new applications in diagnostics, cell monitoring or drug delivery, even though their interaction with biological molecules such as different proteins has not yet been investigated in detail. In this study, the biological behaviour of SiC USNPs in a medium modeling a living organism was investigated in detail through the dependence of the fluorescence on interactions between bovine serum albumin (BSA) and SiC USNPs. The interaction shows transient nanoparticle-protein associations due to the restricted diffusion behaviour of the nanoparticles in the vicinity of a protein. The transient association manifests in a complex fluorescence quenching mechanism where the dynamic component was dominated by Förster resonance energy transfer. By studying SiC nanoparticles of different sizes, it can be concluded that the transient effect is an ultrasmall nanoparticle behaviour.

UR - http://www.scopus.com/inward/record.url?scp=85047470599&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047470599&partnerID=8YFLogxK

U2 - 10.1039/c8cp02144a

DO - 10.1039/c8cp02144a

M3 - Article

C2 - 29722404

AN - SCOPUS:85047470599

VL - 20

SP - 13419

EP - 13429

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

SN - 1463-9076

IS - 19

ER -