Identification of the adrenoceptor subtypes expressed on GABAergic neurons in the anterior hypothalamic area and rostral zona incerta of GAD65-eGFP transgenic mice

Seung Yub Shin, Jian Hua Yang, Hang Lee, F. Erdélyi, Gábor Szabó, So Yeong Lee, Pan Dong Ryu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

GABA is a major neurotransmitter in the hypothalamus. In particular, neurons in the paraventricular nucleus (PVN) of the hypothalamus receive dense GABAergic inputs from peri-PVN regions. The noradrenergic system has been reported as a modulator of GABAergic transmission to the PVN. Previous electrophysiological and morphological studies support the presence of adrenoceptors on GABAergic neurons innervating the PVN. In this study, we identified three adrenoceptors on GABAergic neurons in the peri-PVN region, focusing on the anterior hypothalamic area (AHA) and rostral zona incerta (ZIr). GABAergic neurons were identified using enhanced green fluorescent protein (eGFP), followed by single cell RT-PCR analysis of the GABA synthetic enzymes, glutamic acid decarboxylase (GAD)65 and/or GAD67. Single cell RT-PCR data revealed the expression of α1A-, α1B- and α2A-adrenoceptor mRNA on GABAergic neurons in AHA and ZIr. Additionally, immunohistochemical studies showed that the immunoreactivities of α1A-, α1B- and α2A-adrenoceptor were colocalized with eGFP-expressing neurons in AHA and ZIr. The present findings suggest the contribution of adrenoceptors to the modulation of GABAergic neurons in AHA and ZIr.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalNeuroscience Letters
Volume422
Issue number3
DOIs
Publication statusPublished - Jul 18 2007

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Anterior Hypothalamic Nucleus
GABAergic Neurons
Paraventricular Hypothalamic Nucleus
Adrenergic Receptors
Transgenic Mice
gamma-Aminobutyric Acid
Hypothalamus
GABA Modulators
Neurons
Polymerase Chain Reaction
Glutamate Decarboxylase
Neurotransmitter Agents
Zona Incerta
enhanced green fluorescent protein
Messenger RNA
Enzymes

Keywords

  • α
  • α
  • α-Adrenoceptor
  • Peri-PVN area

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Identification of the adrenoceptor subtypes expressed on GABAergic neurons in the anterior hypothalamic area and rostral zona incerta of GAD65-eGFP transgenic mice. / Shin, Seung Yub; Yang, Jian Hua; Lee, Hang; Erdélyi, F.; Szabó, Gábor; Lee, So Yeong; Ryu, Pan Dong.

In: Neuroscience Letters, Vol. 422, No. 3, 18.07.2007, p. 153-157.

Research output: Contribution to journalArticle

Shin, Seung Yub ; Yang, Jian Hua ; Lee, Hang ; Erdélyi, F. ; Szabó, Gábor ; Lee, So Yeong ; Ryu, Pan Dong. / Identification of the adrenoceptor subtypes expressed on GABAergic neurons in the anterior hypothalamic area and rostral zona incerta of GAD65-eGFP transgenic mice. In: Neuroscience Letters. 2007 ; Vol. 422, No. 3. pp. 153-157.
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