Identification of synaptic plasma membrane proteins co-precipitated with fibrillar β-amyloid peptide

Yann Verdier, Emoke Huszár, B. Penke, Zsuzsa Penke, Gary Woffendin, Michaela Scigelova, L. Fülöp, M. Szücs, Katalin Medzihradszky, T. Janáky

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The β-amyloid peptide that is overproduced in Alzheimer's disease rapidly forms fibrils, which are able to interact with various molecular partners. This study aimed to identify abundant synaptosomal proteins binding to the fibrillar -amyloid (fAβ) 1-42. Triton X-100-soluble proteins were extracted from the rat synaptic plasma membrane fraction. Interacting proteins were isolated by co-precipitation with fAβ, or with fibrillar crystallin as a negative control. Protein identification was accomplished (1) by separating the tryptically digested peptides of the protein pellet by one-dimensional reversed-phase HPLC and analysing them using an ion-trap mass spectrometer with electrospray ionization; and (2) by subjecting the precipitated proteins to gel electrophoretic fractionation, in-gel tryptic digestion and to matrix-assisted laser desorption/ionization time-of-flight mass measurements and post-source decay analysis. Six different synaptosomal proteins co-precipitated with fAβ were identified by both methods: vacuolar proton-pump ATP synthase, glyceraldehyde-3-phosphate dehydrogenase, synapsins I and II, β-tubulin and 2′,3′-cyclic nucleotide 3′-phosphodiesterase. Most of these proteins have already been associated with Alzheimer's disease, and the biological and pathophysiological significance of their interaction with fAβ is discussed.

Original languageEnglish
Pages (from-to)617-628
Number of pages12
JournalJournal of Neurochemistry
Volume94
Issue number3
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Synaptic Membranes
Cell membranes
Amyloid
Blood Proteins
Membrane Proteins
Cell Membrane
Peptides
Synapsins
Proteins
Alzheimer Disease
Gels
Proton Pumps
Crystallins
Glyceraldehyde-3-Phosphate Dehydrogenases
Cyclic Nucleotides
Phosphoric Diester Hydrolases
Octoxynol
Electrospray ionization
Tubulin
Protein Binding

Keywords

  • β-amyloid 1-42
  • Alzheimer's disease
  • Glycolysis
  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
  • Proteins interacting with β-amyloid
  • Synaptic plasma membrane

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Identification of synaptic plasma membrane proteins co-precipitated with fibrillar β-amyloid peptide. / Verdier, Yann; Huszár, Emoke; Penke, B.; Penke, Zsuzsa; Woffendin, Gary; Scigelova, Michaela; Fülöp, L.; Szücs, M.; Medzihradszky, Katalin; Janáky, T.

In: Journal of Neurochemistry, Vol. 94, No. 3, 08.2005, p. 617-628.

Research output: Contribution to journalArticle

Verdier, Yann ; Huszár, Emoke ; Penke, B. ; Penke, Zsuzsa ; Woffendin, Gary ; Scigelova, Michaela ; Fülöp, L. ; Szücs, M. ; Medzihradszky, Katalin ; Janáky, T. / Identification of synaptic plasma membrane proteins co-precipitated with fibrillar β-amyloid peptide. In: Journal of Neurochemistry. 2005 ; Vol. 94, No. 3. pp. 617-628.
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