Identification of sympathetic premotor neurons in medullary raphe regions mediating fever and other thermoregulatory functions

Kazuhiro Nakamura, Kiyoshi Matsumura, Thomas Hübschle, Yoshiko Nakamura, Hiroyuki Hioki, Fumino Fujiyama, Z. Boldogkői, Matthias König, Heinz Jürgen Thiel, Rüdiger Gerstberger, Shigeo Kobayashi, Takeshi Kaneko

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

Sympathetic premotor neurons directly control sympathetic preganglionic neurons (SPNs) in the intermediolateral cell column (IML) of the thoracic spinal cord, and many of these premotor neurons are localized in the medulla oblongata. The rostral ventrolateral medulla contains premotor neurons controlling the cardiovascular conditions, whereas rostral medullary raphe regions are a candidate source of sympathetic premotor neurons for thermoregulatory functions. Here, we show that these medullary raphe regions contain putative glutamatergic neurons and that these neurons directly control thermoregulatory SPNs. Neurons expressing vesicular glutamate transporter 3 (VGLUT3) were distributed in the rat medullary raphe regions, including the raphe magnus and rostral raphe pallidus nuclei, and mostly lacked serotonin immunoreactivity. These VGLUT3-positive neurons expressed Fos in response to cold exposure or to central administration of prostaglandin E2, a pyrogenic mediator. Transneuronal retrograde labeling after inoculation of pseudorabies virus into the interscapular brown adipose tissue (BAT) or the tail indicated that those VGLUT3-expressing medullary raphe neurons innervated these thermoregulatory effector organs multisynaptically through SPNs of specific thoracic segments, and microinjection of glutamate into the IML of the BAT-controlling segments produced BAT thermogenesis. An anterograde tracing study further showed a direct projection of those VGLUT3-expressing medullary raphe neurons to the dendrites of SPNs. Furthermore, intra-IML application of glutamate receptor antagonists blocked BAT thermogenesis triggered by disinhibition of the medullary raphe regions. The present results suggest that VGLUT3-expressing neurons in the medullary raphe regions constitute excitatory neurons that could be categorized as a novel group of sympathetic premotor neurons for thermoregulatory functions, including fever.

Original languageEnglish
Pages (from-to)5370-5380
Number of pages11
JournalJournal of Neuroscience
Volume24
Issue number23
DOIs
Publication statusPublished - Jun 9 2004

Fingerprint

Fever
Neurons
Vesicular Glutamate Transport Proteins
Brown Adipose Tissue
Thermogenesis
Thorax
Suid Herpesvirus 1
Excitatory Amino Acid Antagonists
Medulla Oblongata
Microinjections
Dendrites
Dinoprostone
Tail
Glutamic Acid
Serotonin
Spinal Cord

Keywords

  • Fever
  • Glutamate
  • Premotor neuron
  • Prostaglandin E
  • Raphe
  • Sympathetic nervous system
  • Thermoregulation
  • Vesicular glutamate transporter

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Identification of sympathetic premotor neurons in medullary raphe regions mediating fever and other thermoregulatory functions. / Nakamura, Kazuhiro; Matsumura, Kiyoshi; Hübschle, Thomas; Nakamura, Yoshiko; Hioki, Hiroyuki; Fujiyama, Fumino; Boldogkői, Z.; König, Matthias; Thiel, Heinz Jürgen; Gerstberger, Rüdiger; Kobayashi, Shigeo; Kaneko, Takeshi.

In: Journal of Neuroscience, Vol. 24, No. 23, 09.06.2004, p. 5370-5380.

Research output: Contribution to journalArticle

Nakamura, K, Matsumura, K, Hübschle, T, Nakamura, Y, Hioki, H, Fujiyama, F, Boldogkői, Z, König, M, Thiel, HJ, Gerstberger, R, Kobayashi, S & Kaneko, T 2004, 'Identification of sympathetic premotor neurons in medullary raphe regions mediating fever and other thermoregulatory functions', Journal of Neuroscience, vol. 24, no. 23, pp. 5370-5380. https://doi.org/10.1523/JNEUROSCI.1219-04.2004
Nakamura, Kazuhiro ; Matsumura, Kiyoshi ; Hübschle, Thomas ; Nakamura, Yoshiko ; Hioki, Hiroyuki ; Fujiyama, Fumino ; Boldogkői, Z. ; König, Matthias ; Thiel, Heinz Jürgen ; Gerstberger, Rüdiger ; Kobayashi, Shigeo ; Kaneko, Takeshi. / Identification of sympathetic premotor neurons in medullary raphe regions mediating fever and other thermoregulatory functions. In: Journal of Neuroscience. 2004 ; Vol. 24, No. 23. pp. 5370-5380.
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