Identification of smooth muscle-derived foam cells in the atherosclerotic plaque of human aorta with monoclonal antibody IIG10

Marina A. Glukhova, Olga I. Ornatsky, Maria G. Frid, Alexander E. Kabakov, Rosa R. Adany, Laszlo Muszbek, Vladimir N. Smirnov

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Abstract

We have developed a monoclonal antibody that specifically interacts with a surface antigen of human fibroblasts and smooth muscle cells. The antibody (antibody IIG10) recognizes a polypeptide of molecular mass 330,000, revealed by immunoblotting in fibroblast and smooth muscle cell extract, but not in vascular endothelial cells, peritoneal macrophages, peripheral blood lymphocytes nor hepatocytes. In tissue sections the antibody stained smooth muscle cells of myometrium, aorta and smaller blood vessels, and fibroblasts of connective tissue. Specificity of the antibody was further confirmed by double staining of aorta sections. Antibody IIG10 was used to identify smooth muscle-derived foam cells in the atherosclerotic plaque of human aorta.

Original languageEnglish
Pages (from-to)657-663
Number of pages7
JournalTissue and Cell
Volume19
Issue number5
DOIs
Publication statusPublished - 1987

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Keywords

  • Smooth muscle cell
  • atherosclerosis
  • fibroblast
  • foam cell
  • monoclonal antibody

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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