Identification of smooth muscle-derived foam cells in the atherosclerotic plaque of human aorta with monoclonal antibody IIG10

Marina A. Glukhova, Olga I. Ornatsky, Maria G. Frid, Alexander E. Kabakov, R. Ádány, L. Muszbek, Vladimir N. Smirnov

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have developed a monoclonal antibody that specifically interacts with a surface antigen of human fibroblasts and smooth muscle cells. The antibody (antibody IIG10) recognizes a polypeptide of molecular mass 330,000, revealed by immunoblotting in fibroblast and smooth muscle cell extract, but not in vascular endothelial cells, peritoneal macrophages, peripheral blood lymphocytes nor hepatocytes. In tissue sections the antibody stained smooth muscle cells of myometrium, aorta and smaller blood vessels, and fibroblasts of connective tissue. Specificity of the antibody was further confirmed by double staining of aorta sections. Antibody IIG10 was used to identify smooth muscle-derived foam cells in the atherosclerotic plaque of human aorta.

Original languageEnglish
Pages (from-to)657-663
Number of pages7
JournalTissue and Cell
Volume19
Issue number5
DOIs
Publication statusPublished - 1987

Fingerprint

foam cells
Foam Cells
Atherosclerotic Plaques
aorta
smooth muscle
Smooth Muscle
Aorta
monoclonal antibodies
Monoclonal Antibodies
myocytes
Smooth Muscle Myocytes
fibroblasts
antibodies
Antibodies
Fibroblasts
blood vessels
myometrium
Antibody Specificity
Myometrium
surface antigens

Keywords

  • atherosclerosis
  • fibroblast
  • foam cell
  • monoclonal antibody
  • Smooth muscle cell

ASJC Scopus subject areas

  • Insect Science
  • Cell Biology

Cite this

Identification of smooth muscle-derived foam cells in the atherosclerotic plaque of human aorta with monoclonal antibody IIG10. / Glukhova, Marina A.; Ornatsky, Olga I.; Frid, Maria G.; Kabakov, Alexander E.; Ádány, R.; Muszbek, L.; Smirnov, Vladimir N.

In: Tissue and Cell, Vol. 19, No. 5, 1987, p. 657-663.

Research output: Contribution to journalArticle

Glukhova, Marina A. ; Ornatsky, Olga I. ; Frid, Maria G. ; Kabakov, Alexander E. ; Ádány, R. ; Muszbek, L. ; Smirnov, Vladimir N. / Identification of smooth muscle-derived foam cells in the atherosclerotic plaque of human aorta with monoclonal antibody IIG10. In: Tissue and Cell. 1987 ; Vol. 19, No. 5. pp. 657-663.
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