Identification of site-specific recombination genes int and xis of the Rhizobium temperate phage 16-3

Szabolcs Semsey, Istvan Papp, Zsuzsanna Buzas, Andras Patthy, Laszlo Orosz, Peter P. Papp

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Phage 16-3 is a temperate phage of Rhizobium meliloti 41 which integrates its genome with high efficiency into the host chromosome by site- specific recombination through DNA sequences of attB and attP. Here we report the identification of two phage-encoded genes required for recombinations at these sites: int (phage integration) and xis (prophage excision). We concluded that Int protein of phage 16-3 belongs to the integrase family of tyrosine recombinases. Despite similarities to the cognate systems of the lambdoid phages, the 16-3 int xis att system is not active in Escherichia coli, probably due to requirements for host factors that differ in Rhizobium meliloti and E. coli. The application of the 16-3 site-specific recombination system in biotechnology is discussed.

Original languageEnglish
Pages (from-to)4185-4192
Number of pages8
JournalJournal of bacteriology
Volume181
Issue number14
DOIs
Publication statusPublished - Jul 1999

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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