Identification of site-specific recombination genes int and xis of the Rhizobium temperate phage 16-3

Szabolcs Semsey, I. Papp, Zsuzsanna Buzas, A. Pátthy, L. Orosz, Peter P. Papp

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Phage 16-3 is a temperate phage of Rhizobium meliloti 41 which integrates its genome with high efficiency into the host chromosome by site- specific recombination through DNA sequences of attB and attP. Here we report the identification of two phage-encoded genes required for recombinations at these sites: int (phage integration) and xis (prophage excision). We concluded that Int protein of phage 16-3 belongs to the integrase family of tyrosine recombinases. Despite similarities to the cognate systems of the lambdoid phages, the 16-3 int xis att system is not active in Escherichia coli, probably due to requirements for host factors that differ in Rhizobium meliloti and E. coli. The application of the 16-3 site-specific recombination system in biotechnology is discussed.

Original languageEnglish
Pages (from-to)4185-4192
Number of pages8
JournalJournal of Bacteriology
Volume181
Issue number14
Publication statusPublished - Jul 1999

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Rhizobium
Bacteriophages
Genetic Recombination
Genes
Sinorhizobium meliloti
Escherichia coli
Virus Activation
Integrases
Recombinases
Biotechnology
Tyrosine
Chromosomes
Genome
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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Identification of site-specific recombination genes int and xis of the Rhizobium temperate phage 16-3. / Semsey, Szabolcs; Papp, I.; Buzas, Zsuzsanna; Pátthy, A.; Orosz, L.; Papp, Peter P.

In: Journal of Bacteriology, Vol. 181, No. 14, 07.1999, p. 4185-4192.

Research output: Contribution to journalArticle

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