Identification of positive and negative regulatory regions controlling expression of the cartilage matrix protein gene

I. Kiss, Z. Bösze, Piroska Szabó, Rencendorj Altanchimeg, E. Barta, Ferenc Deák

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A complex pattern of regulation of the cartilage matrix protein gene was revealed by transient expression experiments. A minimal promoter from positions -15 to +64 functioned in chondrocytes and fibroblasts. An enhancer located in the first intron exerted chondrocyte-specific stimulation on the minimal promoter activity. The same fragment, however, had a negative effect in fibroblasts. Between -334 and -15, a silencer was found which inhibited the gene expression driven from its homologous as well as heterologous promoters both in chondrocytes and fibroblasts. Additional positive and negative control regions were mapped further upstream of the promoter.

Original languageEnglish
Pages (from-to)2432-2436
Number of pages5
JournalMolecular and Cellular Biology
Volume10
Issue number5
Publication statusPublished - 1990

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Matrilin Proteins
Nucleic Acid Regulatory Sequences
Chondrocytes
Fibroblasts
Genes
Introns
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Identification of positive and negative regulatory regions controlling expression of the cartilage matrix protein gene. / Kiss, I.; Bösze, Z.; Szabó, Piroska; Altanchimeg, Rencendorj; Barta, E.; Deák, Ferenc.

In: Molecular and Cellular Biology, Vol. 10, No. 5, 1990, p. 2432-2436.

Research output: Contribution to journalArticle

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