Identification of PER-1 extended-spectrum β-lactamase producing Pseudomonas aeruginosa clinical isolates of the international clonal complex CC11 from Hungary and Serbia

B. Libisch, Laurent Poirel, Zorica Lepsanovic, Veljko Mirovic, Boglárka Balogh, J. Pászti, Zsuzsanna Hunyadi, András Dobák, M. Füzi, Patrice Nordmann

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

PER-1 extended-spectrum β-lactamase-producing Pseudomonas aeruginosa clinical isolates from Budapest, Hungary, and Belgrade, Serbia, were characterized by molecular methods. Two PER-1-positive isolates were recovered from sporadic cases in Budapest and a small cluster of PER-1-positive infections involving four patients were identified at a Belgrade hospital. A class 1 integron harbouring a blaOXA-2 β-lactamase gene and four other gene cassettes was detected in both the Budapest and the Belgrade isolates. The two P. aeruginosa isolates from Budapest also carried another class 1 integron containing blaOXA-74, aac(6′)-Ib-cr and cmlA7 genes in its variable region. The aac(6′)-Ib-cr fluoroquinolone-acetylating aminoglycoside acetyltransferase gene is described here for the first time in P. aeruginosa. Multilocus sequence typing (MLST) revealed that the PER-1 positive P. aeruginosa isolates identified in this study display ST235, a sequence type that belongs to clonal complex CC11. Two blaPER-1-positive P. aeruginosa reference isolates from France and Belgium could also be assigned to complex CC11 by MLST. Our results underscore the role of complex CC11 in the dissemination of blaPER-1 among P. aeruginosa clinical isolates.

Original languageEnglish
Pages (from-to)330-338
Number of pages9
JournalFEMS Immunology and Medical Microbiology
Volume54
Issue number3
DOIs
Publication statusPublished - Dec 2008

Fingerprint

Serbia
Hungary
Pseudomonas aeruginosa
Integrons
Multilocus Sequence Typing
Genes
Belgium
Fluoroquinolones
France
Infection

Keywords

  • CC11
  • Fluroquinolone acetyltransferase
  • MLST
  • OXA-74
  • PER-1
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Infectious Diseases
  • Microbiology (medical)
  • Microbiology

Cite this

Identification of PER-1 extended-spectrum β-lactamase producing Pseudomonas aeruginosa clinical isolates of the international clonal complex CC11 from Hungary and Serbia. / Libisch, B.; Poirel, Laurent; Lepsanovic, Zorica; Mirovic, Veljko; Balogh, Boglárka; Pászti, J.; Hunyadi, Zsuzsanna; Dobák, András; Füzi, M.; Nordmann, Patrice.

In: FEMS Immunology and Medical Microbiology, Vol. 54, No. 3, 12.2008, p. 330-338.

Research output: Contribution to journalArticle

Libisch, B. ; Poirel, Laurent ; Lepsanovic, Zorica ; Mirovic, Veljko ; Balogh, Boglárka ; Pászti, J. ; Hunyadi, Zsuzsanna ; Dobák, András ; Füzi, M. ; Nordmann, Patrice. / Identification of PER-1 extended-spectrum β-lactamase producing Pseudomonas aeruginosa clinical isolates of the international clonal complex CC11 from Hungary and Serbia. In: FEMS Immunology and Medical Microbiology. 2008 ; Vol. 54, No. 3. pp. 330-338.
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