Identification of oxytocin and vasopressin from neurohypophyseal cell culture

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23 Citations (Scopus)

Abstract

Our observation that dispersed cultures of neurohypophysis obtained from adult rats are capable of synthesizing and releasing oxytocin and vasopressin is unexpected, because in whole animals these hormones are known only to be stored, not to be produced in the posterior lobe of the pituitary. The hormone content of cell culture medium was elevated from 0 to 129 ± 14 pg/mg protein for oxytocin and from 0 to 42 ± 4 pg/mg protein for vasopressin during two weeks as determined by specific radio-immunoassay. By molecular mass and structure determination (tandem mass spectrometry) we have proved that the supernatant of the cell cultures contains not only immunologically but mass spectrometrically identified neurohypophyseal hormones.

Original languageEnglish
Pages (from-to)1765-1768
Number of pages4
JournalRapid Communications in Mass Spectrometry
Volume12
Issue number22
Publication statusPublished - 1998

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Oxytocin
Vasopressins
Cell culture
Hormones
Posterior Pituitary Hormones
Molecular mass
Molecular structure
Mass spectrometry
Culture Media
Rats
Animals
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

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abstract = "Our observation that dispersed cultures of neurohypophysis obtained from adult rats are capable of synthesizing and releasing oxytocin and vasopressin is unexpected, because in whole animals these hormones are known only to be stored, not to be produced in the posterior lobe of the pituitary. The hormone content of cell culture medium was elevated from 0 to 129 ± 14 pg/mg protein for oxytocin and from 0 to 42 ± 4 pg/mg protein for vasopressin during two weeks as determined by specific radio-immunoassay. By molecular mass and structure determination (tandem mass spectrometry) we have proved that the supernatant of the cell cultures contains not only immunologically but mass spectrometrically identified neurohypophyseal hormones.",
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AU - Janáky, T.

AU - Szabó, P.

AU - Kele, Z.

AU - Baláspiri, Lajos

AU - Varga, C.

AU - Gálfi, M.

AU - Vecsernyés, M.

AU - Gáspár, László

AU - Juhász, A.

AU - László, F.

PY - 1998

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AB - Our observation that dispersed cultures of neurohypophysis obtained from adult rats are capable of synthesizing and releasing oxytocin and vasopressin is unexpected, because in whole animals these hormones are known only to be stored, not to be produced in the posterior lobe of the pituitary. The hormone content of cell culture medium was elevated from 0 to 129 ± 14 pg/mg protein for oxytocin and from 0 to 42 ± 4 pg/mg protein for vasopressin during two weeks as determined by specific radio-immunoassay. By molecular mass and structure determination (tandem mass spectrometry) we have proved that the supernatant of the cell cultures contains not only immunologically but mass spectrometrically identified neurohypophyseal hormones.

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