Identification of novel lumbricin homologues in Eisenia andrei earthworms

Kornélia Bodó, Ákos Boros, Éva Rumpler, László Molnár, Katalin Böröcz, P. Németh, P. Engelmann

Research output: Contribution to journalArticle

  • 1 Citations

Abstract

Lumbricin and its orthologue antimicrobial peptides were typically isolated from annelids. In this report, mRNA for lumbricin and -serendipitously- a novel lumbricin-related mRNA sequence were identified in Eisenia andrei earthworms. The determined mRNA sequences of E. andrei lumbricin and lumbricin-related peptide consist of 477 and 575 nucleotides. The precursors of proline-rich E. andrei lumbricin and the related peptide contain 63 and 59 amino acids, respectively. Phylogenetic analysis indicated close relationship with other annelid lumbricins. Highest expression of both mRNAs appeared in the proximal part of the intestine (pharynx, gizzard), while other tested organs had moderate (body wall, midgut, ovary, metanephridium, seminal vesicles, ventral nerve cord) or low (coelomocytes) levels. During ontogenesis their expression revealed continuous increase in embryos. Following 48 h of in vivo Gram-positive bacteria challenge both mRNAs were significantly elevated in coelomocytes, while Gram-negative bacteria or zymosan stimulation had no detectable effects.

LanguageEnglish
Pages41-46
Number of pages6
JournalDevelopmental and Comparative Immunology
Volume90
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Oligochaeta
Messenger RNA
Peptides
Zymosan
Seminal Vesicles
Gram-Positive Bacteria
Pharynx
Gram-Negative Bacteria
Proline
Intestines
Ovary
Embryonic Structures
Nucleotides
Amino Acids

Keywords

  • Antimicrobial peptides
  • Earthworms
  • Gene expression
  • Innate immunity
  • Lumbricin

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

Identification of novel lumbricin homologues in Eisenia andrei earthworms. / Bodó, Kornélia; Boros, Ákos; Rumpler, Éva; Molnár, László; Böröcz, Katalin; Németh, P.; Engelmann, P.

In: Developmental and Comparative Immunology, Vol. 90, 01.01.2019, p. 41-46.

Research output: Contribution to journalArticle

Bodó, Kornélia ; Boros, Ákos ; Rumpler, Éva ; Molnár, László ; Böröcz, Katalin ; Németh, P. ; Engelmann, P. / Identification of novel lumbricin homologues in Eisenia andrei earthworms. In: Developmental and Comparative Immunology. 2019 ; Vol. 90. pp. 41-46.
@article{b88c34e07721410284ad5523fc3e4d9a,
title = "Identification of novel lumbricin homologues in Eisenia andrei earthworms",
abstract = "Lumbricin and its orthologue antimicrobial peptides were typically isolated from annelids. In this report, mRNA for lumbricin and -serendipitously- a novel lumbricin-related mRNA sequence were identified in Eisenia andrei earthworms. The determined mRNA sequences of E. andrei lumbricin and lumbricin-related peptide consist of 477 and 575 nucleotides. The precursors of proline-rich E. andrei lumbricin and the related peptide contain 63 and 59 amino acids, respectively. Phylogenetic analysis indicated close relationship with other annelid lumbricins. Highest expression of both mRNAs appeared in the proximal part of the intestine (pharynx, gizzard), while other tested organs had moderate (body wall, midgut, ovary, metanephridium, seminal vesicles, ventral nerve cord) or low (coelomocytes) levels. During ontogenesis their expression revealed continuous increase in embryos. Following 48 h of in vivo Gram-positive bacteria challenge both mRNAs were significantly elevated in coelomocytes, while Gram-negative bacteria or zymosan stimulation had no detectable effects.",
keywords = "Antimicrobial peptides, Earthworms, Gene expression, Innate immunity, Lumbricin",
author = "Korn{\'e}lia Bod{\'o} and {\'A}kos Boros and {\'E}va Rumpler and L{\'a}szl{\'o} Moln{\'a}r and Katalin B{\"o}r{\"o}cz and P. N{\'e}meth and P. Engelmann",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.dci.2018.09.001",
language = "English",
volume = "90",
pages = "41--46",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Identification of novel lumbricin homologues in Eisenia andrei earthworms

AU - Bodó, Kornélia

AU - Boros, Ákos

AU - Rumpler, Éva

AU - Molnár, László

AU - Böröcz, Katalin

AU - Németh, P.

AU - Engelmann, P.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Lumbricin and its orthologue antimicrobial peptides were typically isolated from annelids. In this report, mRNA for lumbricin and -serendipitously- a novel lumbricin-related mRNA sequence were identified in Eisenia andrei earthworms. The determined mRNA sequences of E. andrei lumbricin and lumbricin-related peptide consist of 477 and 575 nucleotides. The precursors of proline-rich E. andrei lumbricin and the related peptide contain 63 and 59 amino acids, respectively. Phylogenetic analysis indicated close relationship with other annelid lumbricins. Highest expression of both mRNAs appeared in the proximal part of the intestine (pharynx, gizzard), while other tested organs had moderate (body wall, midgut, ovary, metanephridium, seminal vesicles, ventral nerve cord) or low (coelomocytes) levels. During ontogenesis their expression revealed continuous increase in embryos. Following 48 h of in vivo Gram-positive bacteria challenge both mRNAs were significantly elevated in coelomocytes, while Gram-negative bacteria or zymosan stimulation had no detectable effects.

AB - Lumbricin and its orthologue antimicrobial peptides were typically isolated from annelids. In this report, mRNA for lumbricin and -serendipitously- a novel lumbricin-related mRNA sequence were identified in Eisenia andrei earthworms. The determined mRNA sequences of E. andrei lumbricin and lumbricin-related peptide consist of 477 and 575 nucleotides. The precursors of proline-rich E. andrei lumbricin and the related peptide contain 63 and 59 amino acids, respectively. Phylogenetic analysis indicated close relationship with other annelid lumbricins. Highest expression of both mRNAs appeared in the proximal part of the intestine (pharynx, gizzard), while other tested organs had moderate (body wall, midgut, ovary, metanephridium, seminal vesicles, ventral nerve cord) or low (coelomocytes) levels. During ontogenesis their expression revealed continuous increase in embryos. Following 48 h of in vivo Gram-positive bacteria challenge both mRNAs were significantly elevated in coelomocytes, while Gram-negative bacteria or zymosan stimulation had no detectable effects.

KW - Antimicrobial peptides

KW - Earthworms

KW - Gene expression

KW - Innate immunity

KW - Lumbricin

UR - http://www.scopus.com/inward/record.url?scp=85053078842&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053078842&partnerID=8YFLogxK

U2 - 10.1016/j.dci.2018.09.001

DO - 10.1016/j.dci.2018.09.001

M3 - Article

VL - 90

SP - 41

EP - 46

JO - Developmental and Comparative Immunology

T2 - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

ER -