Identification of novel lumbricin homologues in Eisenia andrei earthworms

Kornélia Bodó, Ákos Boros, Éva Rumpler, László Molnár, Katalin Böröcz, P. Németh, P. Engelmann

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4 Citations (Scopus)

Abstract

Lumbricin and its orthologue antimicrobial peptides were typically isolated from annelids. In this report, mRNA for lumbricin and -serendipitously- a novel lumbricin-related mRNA sequence were identified in Eisenia andrei earthworms. The determined mRNA sequences of E. andrei lumbricin and lumbricin-related peptide consist of 477 and 575 nucleotides. The precursors of proline-rich E. andrei lumbricin and the related peptide contain 63 and 59 amino acids, respectively. Phylogenetic analysis indicated close relationship with other annelid lumbricins. Highest expression of both mRNAs appeared in the proximal part of the intestine (pharynx, gizzard), while other tested organs had moderate (body wall, midgut, ovary, metanephridium, seminal vesicles, ventral nerve cord) or low (coelomocytes) levels. During ontogenesis their expression revealed continuous increase in embryos. Following 48 h of in vivo Gram-positive bacteria challenge both mRNAs were significantly elevated in coelomocytes, while Gram-negative bacteria or zymosan stimulation had no detectable effects.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalDevelopmental and Comparative Immunology
Volume90
DOIs
Publication statusPublished - Jan 1 2019

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Keywords

  • Antimicrobial peptides
  • Earthworms
  • Gene expression
  • Innate immunity
  • Lumbricin

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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