Identification of Hepatozoon erhardovae Krampitz, 1964 from bank voles (Myodes glareolus) and fleas in Southern Hungary

Krisztina Rigó, Gábor Majoros, Sándor Szekeres, Imola Molnár, Mónika Jablonszky, Viktória Majláthová, Igor Majláth, G. Földvári

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In order to investigate the prevalence and life cycle of apicomplexan parasites, small mammals were live-trapped with modified Sherman traps in Southern Hungary between 2010 and 2012. Altogether, 528 rodents (Apodemus flavicollis Melchior, 1834, Apodemus agrarius Pallas, 1771, Myodes glareolus Schreber, 1780, Microtus agrestis Linnaeus, 1761, Mus musculus Linnaeus, 1758 and Micromys minutus Pallas, 1771) were collected and four shrews (Sorex spp.) were by-catched. Captured animals belonging to non-protected species were euthanized, and spleen samples were preserved for histological and molecular analyses. During the examination of spleen smears, Hepatozoon parasites were observed in eight out of 48 bank voles (M. glareolus). DNA was isolated from altogether 221 spleen samples, and 18S rDNA was amplified using two different PCR protocols. The eight bank vole samples were positive with PCR, but none of the other M. glareolus spleen samples or any of the tissue samples from other species were found to be infected. Sequenced amplicons were very similar to Hepatozoon spp. detected in M. glareolus in Spain and Poland. Ectoparasites were collected from the small mammal carcasses and from the vegetation. Hepatozoon DNA was not found in the 181 ticks removed from the small mammals or in the 162 ticks collected with flagging, but was detected in all three flea species (4/43 Megabothris turbidus Rothschild, 1909, 3/10 Ctenophthalmus assimilis Taschenberg, 1880 and 7/78 Ctenophthalmus agyrtes Heller, 1896). Based on gamont morphology, vertebrate and arthropod host species and DNA sequences, the parasites in our study can be identified as Hepatozoon erhardovae.

Original languageEnglish
Pages (from-to)2409-2413
Number of pages5
JournalParasitology Research
Volume115
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Hepatozoon
Siphonaptera
Arvicolinae
Clethrionomys glareolus
Hungary
Spleen
Murinae
Mammals
Parasites
Ctenophthalmus
spleen
Ticks
small mammals
Shrews
parasites
Polymerase Chain Reaction
Megabothris
ticks
Arthropods
DNA

Keywords

  • Bank vole
  • Flea
  • Hepatozoon erhardovae
  • Hungary
  • Myodes glareolus
  • Rodent

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Insect Science
  • veterinary(all)

Cite this

Identification of Hepatozoon erhardovae Krampitz, 1964 from bank voles (Myodes glareolus) and fleas in Southern Hungary. / Rigó, Krisztina; Majoros, Gábor; Szekeres, Sándor; Molnár, Imola; Jablonszky, Mónika; Majláthová, Viktória; Majláth, Igor; Földvári, G.

In: Parasitology Research, Vol. 115, No. 6, 01.06.2016, p. 2409-2413.

Research output: Contribution to journalArticle

Rigó, K, Majoros, G, Szekeres, S, Molnár, I, Jablonszky, M, Majláthová, V, Majláth, I & Földvári, G 2016, 'Identification of Hepatozoon erhardovae Krampitz, 1964 from bank voles (Myodes glareolus) and fleas in Southern Hungary', Parasitology Research, vol. 115, no. 6, pp. 2409-2413. https://doi.org/10.1007/s00436-016-4992-7
Rigó, Krisztina ; Majoros, Gábor ; Szekeres, Sándor ; Molnár, Imola ; Jablonszky, Mónika ; Majláthová, Viktória ; Majláth, Igor ; Földvári, G. / Identification of Hepatozoon erhardovae Krampitz, 1964 from bank voles (Myodes glareolus) and fleas in Southern Hungary. In: Parasitology Research. 2016 ; Vol. 115, No. 6. pp. 2409-2413.
@article{1ed9e918448945e38a8726a7ee982224,
title = "Identification of Hepatozoon erhardovae Krampitz, 1964 from bank voles (Myodes glareolus) and fleas in Southern Hungary",
abstract = "In order to investigate the prevalence and life cycle of apicomplexan parasites, small mammals were live-trapped with modified Sherman traps in Southern Hungary between 2010 and 2012. Altogether, 528 rodents (Apodemus flavicollis Melchior, 1834, Apodemus agrarius Pallas, 1771, Myodes glareolus Schreber, 1780, Microtus agrestis Linnaeus, 1761, Mus musculus Linnaeus, 1758 and Micromys minutus Pallas, 1771) were collected and four shrews (Sorex spp.) were by-catched. Captured animals belonging to non-protected species were euthanized, and spleen samples were preserved for histological and molecular analyses. During the examination of spleen smears, Hepatozoon parasites were observed in eight out of 48 bank voles (M. glareolus). DNA was isolated from altogether 221 spleen samples, and 18S rDNA was amplified using two different PCR protocols. The eight bank vole samples were positive with PCR, but none of the other M. glareolus spleen samples or any of the tissue samples from other species were found to be infected. Sequenced amplicons were very similar to Hepatozoon spp. detected in M. glareolus in Spain and Poland. Ectoparasites were collected from the small mammal carcasses and from the vegetation. Hepatozoon DNA was not found in the 181 ticks removed from the small mammals or in the 162 ticks collected with flagging, but was detected in all three flea species (4/43 Megabothris turbidus Rothschild, 1909, 3/10 Ctenophthalmus assimilis Taschenberg, 1880 and 7/78 Ctenophthalmus agyrtes Heller, 1896). Based on gamont morphology, vertebrate and arthropod host species and DNA sequences, the parasites in our study can be identified as Hepatozoon erhardovae.",
keywords = "Bank vole, Flea, Hepatozoon erhardovae, Hungary, Myodes glareolus, Rodent",
author = "Krisztina Rig{\'o} and G{\'a}bor Majoros and S{\'a}ndor Szekeres and Imola Moln{\'a}r and M{\'o}nika Jablonszky and Vikt{\'o}ria Majl{\'a}thov{\'a} and Igor Majl{\'a}th and G. F{\"o}ldv{\'a}ri",
year = "2016",
month = "6",
day = "1",
doi = "10.1007/s00436-016-4992-7",
language = "English",
volume = "115",
pages = "2409--2413",
journal = "Zeitschrift fur Parasitenkunde",
issn = "0044-3255",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Identification of Hepatozoon erhardovae Krampitz, 1964 from bank voles (Myodes glareolus) and fleas in Southern Hungary

AU - Rigó, Krisztina

AU - Majoros, Gábor

AU - Szekeres, Sándor

AU - Molnár, Imola

AU - Jablonszky, Mónika

AU - Majláthová, Viktória

AU - Majláth, Igor

AU - Földvári, G.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - In order to investigate the prevalence and life cycle of apicomplexan parasites, small mammals were live-trapped with modified Sherman traps in Southern Hungary between 2010 and 2012. Altogether, 528 rodents (Apodemus flavicollis Melchior, 1834, Apodemus agrarius Pallas, 1771, Myodes glareolus Schreber, 1780, Microtus agrestis Linnaeus, 1761, Mus musculus Linnaeus, 1758 and Micromys minutus Pallas, 1771) were collected and four shrews (Sorex spp.) were by-catched. Captured animals belonging to non-protected species were euthanized, and spleen samples were preserved for histological and molecular analyses. During the examination of spleen smears, Hepatozoon parasites were observed in eight out of 48 bank voles (M. glareolus). DNA was isolated from altogether 221 spleen samples, and 18S rDNA was amplified using two different PCR protocols. The eight bank vole samples were positive with PCR, but none of the other M. glareolus spleen samples or any of the tissue samples from other species were found to be infected. Sequenced amplicons were very similar to Hepatozoon spp. detected in M. glareolus in Spain and Poland. Ectoparasites were collected from the small mammal carcasses and from the vegetation. Hepatozoon DNA was not found in the 181 ticks removed from the small mammals or in the 162 ticks collected with flagging, but was detected in all three flea species (4/43 Megabothris turbidus Rothschild, 1909, 3/10 Ctenophthalmus assimilis Taschenberg, 1880 and 7/78 Ctenophthalmus agyrtes Heller, 1896). Based on gamont morphology, vertebrate and arthropod host species and DNA sequences, the parasites in our study can be identified as Hepatozoon erhardovae.

AB - In order to investigate the prevalence and life cycle of apicomplexan parasites, small mammals were live-trapped with modified Sherman traps in Southern Hungary between 2010 and 2012. Altogether, 528 rodents (Apodemus flavicollis Melchior, 1834, Apodemus agrarius Pallas, 1771, Myodes glareolus Schreber, 1780, Microtus agrestis Linnaeus, 1761, Mus musculus Linnaeus, 1758 and Micromys minutus Pallas, 1771) were collected and four shrews (Sorex spp.) were by-catched. Captured animals belonging to non-protected species were euthanized, and spleen samples were preserved for histological and molecular analyses. During the examination of spleen smears, Hepatozoon parasites were observed in eight out of 48 bank voles (M. glareolus). DNA was isolated from altogether 221 spleen samples, and 18S rDNA was amplified using two different PCR protocols. The eight bank vole samples were positive with PCR, but none of the other M. glareolus spleen samples or any of the tissue samples from other species were found to be infected. Sequenced amplicons were very similar to Hepatozoon spp. detected in M. glareolus in Spain and Poland. Ectoparasites were collected from the small mammal carcasses and from the vegetation. Hepatozoon DNA was not found in the 181 ticks removed from the small mammals or in the 162 ticks collected with flagging, but was detected in all three flea species (4/43 Megabothris turbidus Rothschild, 1909, 3/10 Ctenophthalmus assimilis Taschenberg, 1880 and 7/78 Ctenophthalmus agyrtes Heller, 1896). Based on gamont morphology, vertebrate and arthropod host species and DNA sequences, the parasites in our study can be identified as Hepatozoon erhardovae.

KW - Bank vole

KW - Flea

KW - Hepatozoon erhardovae

KW - Hungary

KW - Myodes glareolus

KW - Rodent

UR - http://www.scopus.com/inward/record.url?scp=84961855256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961855256&partnerID=8YFLogxK

U2 - 10.1007/s00436-016-4992-7

DO - 10.1007/s00436-016-4992-7

M3 - Article

C2 - 27003406

AN - SCOPUS:84961855256

VL - 115

SP - 2409

EP - 2413

JO - Zeitschrift fur Parasitenkunde

JF - Zeitschrift fur Parasitenkunde

SN - 0044-3255

IS - 6

ER -