Identification of genes involved in ripening of octoploid strawberry (Fragaria x ananassa Duch.) using cDNA-AFLP

A. Balogh, E. Kiss, T. Koncz, L. Heszky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Strawberry is a typical non-climacteric fruit, its ripening is not well understood yet, particularly the role of ethylene is unclear. In climacteric fruits, ethylene sets up the process of ripening by activation of ripening-specific gene transcription. Ethylene is present in strawberry too, but its effect is not known yet. Therefore, our aims were to identify genes involved in ripening and ethylene-synthesis, their isolation and characterization in strawberry. For this purpose cDNA-AFLP strategy was used which is able to identify genes showing different expression level. It does not need previous sequence information; it is sensitive, reproducible and quantifiable. This technique is suitable for identifying novel genes and differentiating between homologues. The fruit material (Fragaria x ananassa Duch. cv. 'Elsanta') represent four different ripening stages (green, white, pink and fully ripe) with and without 1-MCP (1-methyl-cyclopropene) treatment. 1-MCP binds specifically to the ethylene receptors blocking the ethylene signal transduction pathway, so it is suitable to find genes indirectly, activated by ethylene. The 270 cDNA fragments amplified by cDNA-AFLP method showed significant difference in expression pattern, according to ripening stage and tissue type. The cDNA fragments were grouped by Cluster and TreeView programs into three categories: genes expressing in the achenes, in the green receptacles and the ripe (red) receptacle. After statistical analysis, cDNA fragments were isolated and re-amplified by PCR. The sequences were compared to database sequences to find homology. Sequence analysis of cDNA fragments showed, that some of the cDNAs represent genes having key role in ripening, their expression pattern is similar to that of climacteric fruits. Other genes - including genes functioning in the achene - code for proteins: storage, allergic and heat-shock ones.

Original languageEnglish
Title of host publicationActa Horticulturae
Pages635-641
Number of pages7
Volume725 II
Publication statusPublished - Nov 2006

Publication series

NameActa Horticulturae
Volume725 II
ISSN (Print)05677572

Fingerprint

Fragaria ananassa
strawberries
amplified fragment length polymorphism
ripening
ethylene
genes
fruits
1-methylcyclopropene
storage proteins
heat stress
signal transduction
statistical analysis
sequence analysis
transcription (genetics)
receptors
synthesis

Keywords

  • 1-MCP
  • cDNA-AFLP
  • Gene expression
  • Ripening
  • Strawberry

ASJC Scopus subject areas

  • Horticulture

Cite this

Balogh, A., Kiss, E., Koncz, T., & Heszky, L. (2006). Identification of genes involved in ripening of octoploid strawberry (Fragaria x ananassa Duch.) using cDNA-AFLP. In Acta Horticulturae (Vol. 725 II, pp. 635-641). (Acta Horticulturae; Vol. 725 II).

Identification of genes involved in ripening of octoploid strawberry (Fragaria x ananassa Duch.) using cDNA-AFLP. / Balogh, A.; Kiss, E.; Koncz, T.; Heszky, L.

Acta Horticulturae. Vol. 725 II 2006. p. 635-641 (Acta Horticulturae; Vol. 725 II).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Balogh, A, Kiss, E, Koncz, T & Heszky, L 2006, Identification of genes involved in ripening of octoploid strawberry (Fragaria x ananassa Duch.) using cDNA-AFLP. in Acta Horticulturae. vol. 725 II, Acta Horticulturae, vol. 725 II, pp. 635-641.
Balogh A, Kiss E, Koncz T, Heszky L. Identification of genes involved in ripening of octoploid strawberry (Fragaria x ananassa Duch.) using cDNA-AFLP. In Acta Horticulturae. Vol. 725 II. 2006. p. 635-641. (Acta Horticulturae).
Balogh, A. ; Kiss, E. ; Koncz, T. ; Heszky, L. / Identification of genes involved in ripening of octoploid strawberry (Fragaria x ananassa Duch.) using cDNA-AFLP. Acta Horticulturae. Vol. 725 II 2006. pp. 635-641 (Acta Horticulturae).
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