Identification of fish-parasitic Myxobolus (Myxosporea) species using a combined PCR-RFLP method

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31 Citations (Scopus)

Abstract

Polymerase chain reaction (PCR) with primers specific for the family Myxobolidae was used to amplify a part of the 18S ribosomal RNA gene of Myxobolus species. The length of the amplified fragments was approximately 1600 base pairs. Six Myxobolus species identified on the basis of morphological features were compared using a combined PCR-RFLP method. The cleavage patterns generated by 2 frequent cutter restriction enzymes (HinfI and MspI) were suitable for the differentiation of the examined Myxobolus species.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalDiseases of Aquatic Organisms
Volume44
Issue number1
DOIs
Publication statusPublished - Jan 26 2001

Keywords

  • Fish parasite
  • Myxobolus spp.
  • Myxosporea
  • PCR
  • RFLP

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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