Identification of factor C protein from Streptomyces griseus by microelectrospray mass spectrometry

Pál T. Szabó, Zoltán Kele, Zsuzsa Birkó, Ferenc Szeszák, Sándor Bíró, Tamás Janáky

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5 Citations (Scopus)

Abstract

Factor C, an extracellular signal protein of cellular differentiation, was studied and significant homology was found to several zinc finger-type regulatory proteins. The complete amino acid sequence, deduced from the gene, that encodes the protein, did not support the hypothesis that this protein might be a zinc finger-type regulatory protein. However, a theoretical single nucleotide insertion in the gene can result in another similarly sized protein containing about 20 His residues, which would be responsible for the high zinc affinity of factor C. The protein sample was reduced, alkylated and then in-gel digested with trypsin. The peptide fragments were then separated by capillary chromatography and identified by microelectrospray mass spectrometry. Peaks of higher intensity were sequenced by tandem mass spectrometry. The identified peptide fragments and the measured molecular mass of factor C protein also confirmed the original sequence of protein, as there was no shift in the open reading frame.

Original languageEnglish
Pages (from-to)1312-1316
Number of pages5
JournalJournal of Mass Spectrometry
Volume34
Issue number12
DOIs
Publication statusPublished - Dec 1 1999

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Keywords

  • Capillary chromatography
  • Factor c
  • Microelectrospray mass spectrometry
  • Sequence identification
  • Streptomyces

ASJC Scopus subject areas

  • Spectroscopy

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