Identification of DUOX1-dependent redox signaling through protein S-glutathionylation in airway epithelial cells

Milena Hristova, Carmen Veith, Aida Habibovic, Ying Wai Lam, Bin Deng, Miklos Geiszt, Yvonne M.W. Janssen-Heininger, Albert Van der Vliet

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The NADPH oxidase homolog dual oxidase 1 (DUOX1) plays an important role in innate airway epithelial responses to infection or injury, but the precise molecular mechanisms are incompletely understood and the cellular redox-sensitive targets for DUOX1-derived H2O2 have not been identified. The aim of the present study was to survey the involvement of DUOX1 in cellular redox signaling by protein S-glutathionylation, a major mode of reversible redox signaling. Using human airway epithelial H292 cells and stable transfection with DUOX1-targeted shRNA as well as primary tracheal epithelial cells from either wild-type or DUOX1-deficient mice, DUOX1 was found to be critical in ATP-stimulated transient production of H2O2 and increased protein S-glutathionylation. Using cell pre-labeling with biotin-tagged GSH and analysis of avidin-purified proteins by global proteomics, 61 S-glutathionylated proteins were identified in ATP-stimulated cells compared to 19 in untreated cells. Based on a previously established role of DUOX1 in cell migration, various redox-sensitive proteins with established roles in cytoskeletal dynamics and/or cell migration were evaluated for S-glutathionylation, indicating a critical role for DUOX1 in ATP-stimulated S-glutathionylation of β-actin, peroxiredoxin 1, the non-receptor tyrosine kinase Src, and MAPK phosphatase 1. Overall, our studies demonstrate the importance of DUOX1 in epithelial redox signaling through reversible S-glutathionylation of a range of proteins, including proteins involved in cytoskeletal regulation and MAPK signaling pathways involved in cell migration.

Original languageEnglish
Pages (from-to)436-446
Number of pages11
JournalRedox Biology
Volume2
Issue number1
DOIs
Publication statusPublished - Jan 2014

Keywords

  • Cell migration
  • Cysteine
  • DUOX1
  • NADPH oxidase
  • Proteomics
  • S-glutathionylation

ASJC Scopus subject areas

  • Organic Chemistry
  • Clinical Biochemistry

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  • Cite this

    Hristova, M., Veith, C., Habibovic, A., Lam, Y. W., Deng, B., Geiszt, M., Janssen-Heininger, Y. M. W., & Van der Vliet, A. (2014). Identification of DUOX1-dependent redox signaling through protein S-glutathionylation in airway epithelial cells. Redox Biology, 2(1), 436-446. https://doi.org/10.1016/j.redox.2013.12.030