Identification of diverse archaeal proteins with class III signal peptides cleaved by distinct archaeal prepilin peptidases

Z. Szabó, Adriana Oliveira Stahl, Sonja V. Albers, Jessica C. Kissinger, Arnold J M Driessen, Mechthild Pohlschröder

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Most secreted archaeal proteins are targeted to the membrane via a tripartite signal composed of a charged N terminus and a hydrophobic domain, followed by a signal peptidase-processing site. Signal peptides of archaeal flagellins, similar to class III signal peptides of bacterial type IV piling, are distinct in that their processing sites precede the hydrophobic domain, which is crucial for assembly of these estracytoplasmic structures. To identify the complement of archaeal proteins with class III signal sequences, a PERL program (FlaFind) was written. A diverse set of proteins was identified, and many of these FlaFind positives were encoded by genes that were cotranscribed with homologs of pilus assembly genes. Moreover, structural conservation of primary sequences between many FlaFind positives and subunits of bacterial pilus-like structures, which have been shown to be critical for pilin assembly, have been observed. A subset of pilin-like FlaFind positives contained a conserved domain of unknown function (DUF361) within the signal peptide. Many of the genes encoding these proteins were in operons that contained a gene encoding a novel euryarchaeal prepilin-peptidase, EppA, homolog. Heterologous analysis revealed that Methanococcus maripaludis DUF361-containing proteins were specifically processed by the EppA homolog of this archaeon. Conversely, M. maripaludis preflagellins were cleaved only by the archaeal preflagellin peptidase FlaK. Together, the results reveal a diverse set of archaeal proteins with class III signal peptides that might be subunits of as-yet-undescribed cell surface structures, such as archaeal pili.

Original languageEnglish
Pages (from-to)772-778
Number of pages7
JournalJournal of Bacteriology
Volume189
Issue number3
DOIs
Publication statusPublished - Feb 2007

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Archaeal Proteins
Protein Sorting Signals
Peptide Hydrolases
Fimbriae Proteins
Bacterial Fimbriae
Methanococcus
Genes
Flagellin
Proteins
Archaea
Operon
Complement System Proteins
Membranes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Identification of diverse archaeal proteins with class III signal peptides cleaved by distinct archaeal prepilin peptidases. / Szabó, Z.; Stahl, Adriana Oliveira; Albers, Sonja V.; Kissinger, Jessica C.; Driessen, Arnold J M; Pohlschröder, Mechthild.

In: Journal of Bacteriology, Vol. 189, No. 3, 02.2007, p. 772-778.

Research output: Contribution to journalArticle

Szabó, Z. ; Stahl, Adriana Oliveira ; Albers, Sonja V. ; Kissinger, Jessica C. ; Driessen, Arnold J M ; Pohlschröder, Mechthild. / Identification of diverse archaeal proteins with class III signal peptides cleaved by distinct archaeal prepilin peptidases. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 3. pp. 772-778.
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