Identification of COS markers specific for Thinopyrum elongatum chromosomes preliminary revealed high level of macrosyntenic relationship between the wheat and Th. Elongatum genomes

Eszter Gaál, Miroslav Valárik, István Molnár, András Farkas, G. Linc

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Thinopyrum elongatum (Host) D.R. Dewey has served as an important gene source for wheat breeding improvement for many years. The exact characterization of its chromosomes is important for the detailed analysis of prebreeding materials produced with this species. The major aim of this study was to identify and characterize new molecular markers to be used for the rapid analysis of E genome chromatin in wheat background. Sixty of the 169 conserved orthologous set (COS) markers tested on diverse wheat-Th. elongatum disomic/ ditelosomic addition lines were assigned to various Th. elongatum chromosomes and will be used for marker-assisted selection. The macrosyntenic relationship between the wheat and Th. elongatum genomes was investigated using EST sequences. Several rearrangements were revealed in homoeologous chromosome groups 2, 5, 6 and 7, while chromosomes 1 and 4 were conserved. Molecular cytogenetic and marker analysis showed the presence of rearranged chromosome involved in 6ES and 2EL arms in the 6E disomic addition line. The selected chromosome arm-specific COS markers will make it possible to identify gene introgressions in breeding programmes and will also be useful in the development of new chromosome-specific markers, evolutionary analysis and gene mapping.

Original languageEnglish
Article numbere0208840
JournalPLoS One
Volume13
Issue number12
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Thinopyrum elongatum
Chromosomes
Triticum
Genes
Genome
chromosomes
wheat
genome
Breeding
disomics
Chromosomes, Human, Pair 4
Chromosomes, Human, Pair 2
Chromosome Mapping
Chromosomes, Human, Pair 1
Cytogenetic Analysis
Expressed Sequence Tags
Genetic Markers
Chromatin
breeding
cytogenetics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Identification of COS markers specific for Thinopyrum elongatum chromosomes preliminary revealed high level of macrosyntenic relationship between the wheat and Th. Elongatum genomes. / Gaál, Eszter; Valárik, Miroslav; Molnár, István; Farkas, András; Linc, G.

In: PLoS One, Vol. 13, No. 12, e0208840, 01.12.2018.

Research output: Contribution to journalArticle

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