Identification of blaLAP-2 and qnrS1 genes in the internationally successful Klebsiella pneumoniae ST147 clone

B. Kocsis, Erika Kocsis, Roberta Fontana, Giuseppe Cornaglia, Annarita Mazzariol

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We investigated the presence of β-lactamase genes (bla) in 26 strains of Enterobacteriaceae already found positive for the qnrS1 gene, a plasmid-mediated quinolone resistance determinant. Three strains of K. pneumoniae, isolated in the period 2008-2009 at the University Hospital in Verona, were positive for LAP-2, a narrow-spectrum β-lactamase. These strains, namely VRB586, VRE185 and VRE196, were cultured from urine, bile and peritoneal drainage, respectively, of different patients from different units. The blaLAP-2 and qnrS1 resistance determinant genes were separated by ISEcl2 and were located on a 97 kb conjugable and untypable plasmid, which could be transferred to a recipient strain, E. coli J53. The fluoroquinolone and ceftazidime MICs increased 1-2-fold in the transconjugant cells. The three K. pneumoniae strains were found to be clonal by PFGE and were identified as belonging to ST147, an internationally successful clone, by MLST. The plasmid sequence, including ISEcl2 and qnrS1 genes, of K. pneumoniae ST147 was found to be highly similar to previously detected qnrS1-harbouring plasmids, suggesting the plasmid has a stable genetic structure and that these resistance determinants have a common source. To the best of our knowledge, this is the first report of the internationally successful K. pneumoniae ST147 strain carrying blaLAP-2 and qnrS1 genes and is the first case of LAP β-lactamase in Italy.

Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalJournal of Medical Microbiology
Volume62
Issue numberPART 2
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Klebsiella pneumoniae
Plasmids
Clone Cells
Genes
Ceftazidime
Genetic Structures
Quinolones
Fluoroquinolones
Enterobacteriaceae
Bile
Italy
Drainage
Urine
Escherichia coli

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Identification of blaLAP-2 and qnrS1 genes in the internationally successful Klebsiella pneumoniae ST147 clone. / Kocsis, B.; Kocsis, Erika; Fontana, Roberta; Cornaglia, Giuseppe; Mazzariol, Annarita.

In: Journal of Medical Microbiology, Vol. 62, No. PART 2, 02.2013, p. 269-273.

Research output: Contribution to journalArticle

Kocsis, B. ; Kocsis, Erika ; Fontana, Roberta ; Cornaglia, Giuseppe ; Mazzariol, Annarita. / Identification of blaLAP-2 and qnrS1 genes in the internationally successful Klebsiella pneumoniae ST147 clone. In: Journal of Medical Microbiology. 2013 ; Vol. 62, No. PART 2. pp. 269-273.
@article{a1c4b8bfffc54fe889a0ef03de3dc923,
title = "Identification of blaLAP-2 and qnrS1 genes in the internationally successful Klebsiella pneumoniae ST147 clone",
abstract = "We investigated the presence of β-lactamase genes (bla) in 26 strains of Enterobacteriaceae already found positive for the qnrS1 gene, a plasmid-mediated quinolone resistance determinant. Three strains of K. pneumoniae, isolated in the period 2008-2009 at the University Hospital in Verona, were positive for LAP-2, a narrow-spectrum β-lactamase. These strains, namely VRB586, VRE185 and VRE196, were cultured from urine, bile and peritoneal drainage, respectively, of different patients from different units. The blaLAP-2 and qnrS1 resistance determinant genes were separated by ISEcl2 and were located on a 97 kb conjugable and untypable plasmid, which could be transferred to a recipient strain, E. coli J53. The fluoroquinolone and ceftazidime MICs increased 1-2-fold in the transconjugant cells. The three K. pneumoniae strains were found to be clonal by PFGE and were identified as belonging to ST147, an internationally successful clone, by MLST. The plasmid sequence, including ISEcl2 and qnrS1 genes, of K. pneumoniae ST147 was found to be highly similar to previously detected qnrS1-harbouring plasmids, suggesting the plasmid has a stable genetic structure and that these resistance determinants have a common source. To the best of our knowledge, this is the first report of the internationally successful K. pneumoniae ST147 strain carrying blaLAP-2 and qnrS1 genes and is the first case of LAP β-lactamase in Italy.",
author = "B. Kocsis and Erika Kocsis and Roberta Fontana and Giuseppe Cornaglia and Annarita Mazzariol",
year = "2013",
month = "2",
doi = "10.1099/jmm.0.050542-0",
language = "English",
volume = "62",
pages = "269--273",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "PART 2",

}

TY - JOUR

T1 - Identification of blaLAP-2 and qnrS1 genes in the internationally successful Klebsiella pneumoniae ST147 clone

AU - Kocsis, B.

AU - Kocsis, Erika

AU - Fontana, Roberta

AU - Cornaglia, Giuseppe

AU - Mazzariol, Annarita

PY - 2013/2

Y1 - 2013/2

N2 - We investigated the presence of β-lactamase genes (bla) in 26 strains of Enterobacteriaceae already found positive for the qnrS1 gene, a plasmid-mediated quinolone resistance determinant. Three strains of K. pneumoniae, isolated in the period 2008-2009 at the University Hospital in Verona, were positive for LAP-2, a narrow-spectrum β-lactamase. These strains, namely VRB586, VRE185 and VRE196, were cultured from urine, bile and peritoneal drainage, respectively, of different patients from different units. The blaLAP-2 and qnrS1 resistance determinant genes were separated by ISEcl2 and were located on a 97 kb conjugable and untypable plasmid, which could be transferred to a recipient strain, E. coli J53. The fluoroquinolone and ceftazidime MICs increased 1-2-fold in the transconjugant cells. The three K. pneumoniae strains were found to be clonal by PFGE and were identified as belonging to ST147, an internationally successful clone, by MLST. The plasmid sequence, including ISEcl2 and qnrS1 genes, of K. pneumoniae ST147 was found to be highly similar to previously detected qnrS1-harbouring plasmids, suggesting the plasmid has a stable genetic structure and that these resistance determinants have a common source. To the best of our knowledge, this is the first report of the internationally successful K. pneumoniae ST147 strain carrying blaLAP-2 and qnrS1 genes and is the first case of LAP β-lactamase in Italy.

AB - We investigated the presence of β-lactamase genes (bla) in 26 strains of Enterobacteriaceae already found positive for the qnrS1 gene, a plasmid-mediated quinolone resistance determinant. Three strains of K. pneumoniae, isolated in the period 2008-2009 at the University Hospital in Verona, were positive for LAP-2, a narrow-spectrum β-lactamase. These strains, namely VRB586, VRE185 and VRE196, were cultured from urine, bile and peritoneal drainage, respectively, of different patients from different units. The blaLAP-2 and qnrS1 resistance determinant genes were separated by ISEcl2 and were located on a 97 kb conjugable and untypable plasmid, which could be transferred to a recipient strain, E. coli J53. The fluoroquinolone and ceftazidime MICs increased 1-2-fold in the transconjugant cells. The three K. pneumoniae strains were found to be clonal by PFGE and were identified as belonging to ST147, an internationally successful clone, by MLST. The plasmid sequence, including ISEcl2 and qnrS1 genes, of K. pneumoniae ST147 was found to be highly similar to previously detected qnrS1-harbouring plasmids, suggesting the plasmid has a stable genetic structure and that these resistance determinants have a common source. To the best of our knowledge, this is the first report of the internationally successful K. pneumoniae ST147 strain carrying blaLAP-2 and qnrS1 genes and is the first case of LAP β-lactamase in Italy.

UR - http://www.scopus.com/inward/record.url?scp=84872690484&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872690484&partnerID=8YFLogxK

U2 - 10.1099/jmm.0.050542-0

DO - 10.1099/jmm.0.050542-0

M3 - Article

C2 - 23118475

AN - SCOPUS:84872690484

VL - 62

SP - 269

EP - 273

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - PART 2

ER -