Identification of bifucosylated glycoforms using low-energy CID spectra

András Ács, Lilla Turiák, Ágnes Révész, Károly Vékey, László Drahos

Research output: Contribution to journalArticle

Abstract

We have used tandem mass spectrometry (MS/MS)-based analysis of glycopeptides in order to identify the composition and structure of rare glycoforms. The results illustrate utility of low-energy MS/MS for structure identification. We have shown the presence of bifucosylated and trifucosylated glycoforms in human α-1-acid glycoprotein (AGP), a major plasma glycoprotein. Fucosylation in the case of AGP always occurs on the antennae; core fucosylation was not observed.

Original languageEnglish
Pages (from-to)817-822
Number of pages6
JournalJournal of Mass Spectrometry
Volume54
Issue number10
DOIs
Publication statusPublished - Oct 1 2019

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Keywords

  • fucosylation
  • glycopeptide
  • glycosylation
  • low-energy CID

ASJC Scopus subject areas

  • Spectroscopy

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