Identification of astroviruses in bovine and buffalo calves with enteritis

Vito Martella, Cristiana Catella, Paolo Capozza, Georgia Diakoudi, Michele Camero, Gianvito Lanave, Domenico Galante, Maria Assunta Cafiero, Antonio Lavazza, Krisztián Bányai, Canio Buonavoglia

Research output: Contribution to journalArticle

Abstract

Astroviruses (AstVs) have been identified in the stools of calves with enteritis and in the brain tissues of bovines with encephalitis but their pathogenic role has not been clarified. In this study, we report the detection and characterization of bovine and water buffalo AstV strains identified in young bovine and buffalo calves with enteritis in Italy between 2012 and 2015. By negative staining transmission electron microscopy (TEM) observation, AstV-like particles were identified in the stools of the animals and AstV RNA was confirmed molecularly. The sequence (~3.2-kb) at the 3′ end of the genome was determined for two bovine and two buffalo AstVs. Sequence and phylogenetic analysis on the partial ORF1b and full-length ORF2 revealed a marked genetic diversity although the viruses were distantly related to other AstV identified from ruminants. Gathering sequence information on ruminant AstVs is important to understand the extent of inter-species circulation and for the development of reliable, specific diagnostic tools.

Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalResearch in veterinary science
Volume131
DOIs
Publication statusPublished - Aug 2020

Keywords

  • Astrovirus
  • Bovine
  • Buffalo
  • Calves
  • Enteritis

ASJC Scopus subject areas

  • veterinary(all)

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    Martella, V., Catella, C., Capozza, P., Diakoudi, G., Camero, M., Lanave, G., Galante, D., Cafiero, M. A., Lavazza, A., Bányai, K., & Buonavoglia, C. (2020). Identification of astroviruses in bovine and buffalo calves with enteritis. Research in veterinary science, 131, 59-68. https://doi.org/10.1016/j.rvsc.2020.04.010