Identification of an rsh Gene from a Novosphingobium sp. Necessary for Quorum-Sensing Signal Accumulation

Han Ming Gan, Larry Buckley, E. Szegedi, André O. Hudson, Michael A. Savka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The stringent response is a mechanism by which bacteria adapt to environmental stresses and nutritional deficiencies through the synthesis and hydrolysis of (p)ppGpp by RelA/SpoT enzymes. Alphaproteobacteria and plants contain a single Rsh enzyme (named for ifelA/SpoT homolog) that is bifunctional. Here we report the identification of a new species of bacteria belonging to the genus Novosphingobium and characterization of an rsh mutation in this plant tumor-associated isolate. Isolate Rr 2-17, from a grapevine crown gall tumor, is a member of the Novosphingobium genus that produces the N-acyl-homoserine lactone (AHL) quorum-sensing (QS) signals. A Tn5 mutant, Hx 699, deficient in AHL production was found to have an insertion in an rsh gene. The Rsh protein showed significant percent sequence identity to Rsh proteins of alphaproteobacteria. The Novosphingobium sp. rsh gene (rsh Nsp) complemented the multiple amino acid requirements of the Escherichia coli relA spoT double mutant by restoring the growth on selection media. Besides QS signal production, the rsh mutation also affects soluble polysaccharide production and cell aggregation. Genetic complementation of the Hx 699 mutant with the rsh Nsp gene restored these phenotypes. This is the first discovery of a functional rsh gene in a member of the Novosphingobium genus.

Original languageEnglish
Pages (from-to)2551-2560
Number of pages10
JournalJournal of Bacteriology
Volume191
Issue number8
DOIs
Publication statusPublished - Apr 2009

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Quorum Sensing
Plant Tumors
Acyl-Butyrolactones
Alphaproteobacteria
Genes
Bacteria
Cell Aggregation
Mutation
Malnutrition
Polysaccharides
Proteins
Hydrolysis
Escherichia coli
Phenotype
Amino Acids
Enzymes
Growth
Neoplasms

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Identification of an rsh Gene from a Novosphingobium sp. Necessary for Quorum-Sensing Signal Accumulation. / Gan, Han Ming; Buckley, Larry; Szegedi, E.; Hudson, André O.; Savka, Michael A.

In: Journal of Bacteriology, Vol. 191, No. 8, 04.2009, p. 2551-2560.

Research output: Contribution to journalArticle

Gan, Han Ming ; Buckley, Larry ; Szegedi, E. ; Hudson, André O. ; Savka, Michael A. / Identification of an rsh Gene from a Novosphingobium sp. Necessary for Quorum-Sensing Signal Accumulation. In: Journal of Bacteriology. 2009 ; Vol. 191, No. 8. pp. 2551-2560.
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