Identification of acid- and thermotolerant extracellular β-glucosidase activities in Zygomycetes fungi

M. Takó, Elvira Farkas, Szabina Lung, Judit Krisch, Cs Vágvölgyi, T. Papp

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Abstract

Extracellular β-glucosidase activity of 94 strains, representing 24 species of the genera Gilbertella, Mucor, Rhizomucor , and Rhizopus was evaluated in submerged culture and under solid state fermentation on wheat bran. Gilbertella persicaria G1 isolate showed the highest activity (70.9 U ml -1) followed by other Gilbertella (58.6-59.0 U ml-1) and Rhizomucor miehei isolates (29.2-42.0 U ml-1). Optimum temperature for enzyme production was 25 °C for Gilbertella and Mucor , and 30 °C for Rhizomucor and Rhizopus strains. Enzymes of R. miehei strains proved to be thermotolerant preserving up to 92.8% residual activity after heating to 75 °C in the presence of cellobiose substrate. Enzymes of Mucor racemosus f. chibinensis, R. miehei and Rhizopus microsporus var. oligosporus strains were activated at acidic condition (pH 4). Glucose was a strong inhibitor for each fungal β-glucosidase tested but some of them showed ethanol tolerance up to 20% (v/v). Ethanol also activated the enzyme in these strains suggesting glycosyl transferase activity.

Original languageEnglish
Pages (from-to)101-110
Number of pages10
JournalActa biologica Hungarica
Volume61
Issue number1
DOIs
Publication statusPublished - Mar 1 2010

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Keywords

  • Gilbertella
  • Mucor
  • Rhizomucor
  • Rhizopus
  • β-glucosidase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

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