Identification of a splice variant of the metal-responsive transcription factor MTF-1 in common carp

Ágnes Ferencz, E. Hermesz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Transactivation of the expression of metallothionein genes involves the Metal-responsive Transcription Factor (MTF-1). We report here the identification of mtf-1.1a, the first known splice variant of mtf-1.1 mRNA, in common carp (Cyprinus carpio). The lack of a 103 nt internal segment results in a frame shift, causing the early termination of translation. mtf-1.1a mRNA encodes a protein consisting of the first 349 amino acids of MTF-1.1 plus an additional 64 amino acids, with no significant similarity to any of the proteins in the databases. The predicted MTF-1.1a protein carries the Zn-finger domain and the nuclear exporting and nuclear localization signals, and lacks the transcription activation domains. mtf-1.1a was detected in all tissues examined but the liver, with the highest level in the brain. Arsenic alters the levels of both mtf-1.1 and mtf-1.1a transcripts, in an isoform- and tissue-specific manner.

Original languageEnglish
Pages (from-to)113-117
Number of pages5
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume150
Issue number1
DOIs
Publication statusPublished - Jul 2009

Fingerprint

Carps
Transcriptional Activation
Metals
Amino Acids
Nuclear Localization Signals
Protein Databases
Messenger RNA
Metallothionein
Arsenic
Tissue
Fingers
Protein Isoforms
Proteins
Transcription
Gene Expression
Liver
Brain
Genes
Chemical activation
transcription factor MTF-1

Keywords

  • Alternative spicing
  • Arsenic
  • Cadmium
  • Metallothionein
  • mtf-1

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

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abstract = "Transactivation of the expression of metallothionein genes involves the Metal-responsive Transcription Factor (MTF-1). We report here the identification of mtf-1.1a, the first known splice variant of mtf-1.1 mRNA, in common carp (Cyprinus carpio). The lack of a 103 nt internal segment results in a frame shift, causing the early termination of translation. mtf-1.1a mRNA encodes a protein consisting of the first 349 amino acids of MTF-1.1 plus an additional 64 amino acids, with no significant similarity to any of the proteins in the databases. The predicted MTF-1.1a protein carries the Zn-finger domain and the nuclear exporting and nuclear localization signals, and lacks the transcription activation domains. mtf-1.1a was detected in all tissues examined but the liver, with the highest level in the brain. Arsenic alters the levels of both mtf-1.1 and mtf-1.1a transcripts, in an isoform- and tissue-specific manner.",
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AB - Transactivation of the expression of metallothionein genes involves the Metal-responsive Transcription Factor (MTF-1). We report here the identification of mtf-1.1a, the first known splice variant of mtf-1.1 mRNA, in common carp (Cyprinus carpio). The lack of a 103 nt internal segment results in a frame shift, causing the early termination of translation. mtf-1.1a mRNA encodes a protein consisting of the first 349 amino acids of MTF-1.1 plus an additional 64 amino acids, with no significant similarity to any of the proteins in the databases. The predicted MTF-1.1a protein carries the Zn-finger domain and the nuclear exporting and nuclear localization signals, and lacks the transcription activation domains. mtf-1.1a was detected in all tissues examined but the liver, with the highest level in the brain. Arsenic alters the levels of both mtf-1.1 and mtf-1.1a transcripts, in an isoform- and tissue-specific manner.

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