Identification of a Second Poly(A) Polymerase in Escherichia coli

M. Kalapos, G. J. Cao, S. R. Kushner, N. Sarkar

Research output: Contribution to journalArticle

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Abstract

A second poly(A) polymerase (PAP II) has been identified in Escherichia coli using a strain carrying a deletion of pcnB (the structural gene for PAP I; Cao and Sarkar, 1992b) and pnp-7 (a null mutation in the structural gene for polynucleotide phosphorylase). While PAP I has a Mr of 53,000, PAP II is a smaller protein with a native Mr ∼35,000. PAP II differs from PAP I in preferring poly(A) over tRNA primers and being more thermolabile. The presence of multiple poly(A) polymerases in E. coli raises interesting questions regarding the role of polyadenylation in mRNA synthesis and decay.

Original languageEnglish
Pages (from-to)459-465
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume198
Issue number2
DOIs
Publication statusPublished - Jan 31 1994

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Polynucleotide Adenylyltransferase
Escherichia coli
Genes
Polyribonucleotide Nucleotidyltransferase
Polyadenylation
Poly A
RNA Stability
Transfer RNA
Messenger RNA
Mutation
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Identification of a Second Poly(A) Polymerase in Escherichia coli. / Kalapos, M.; Cao, G. J.; Kushner, S. R.; Sarkar, N.

In: Biochemical and Biophysical Research Communications, Vol. 198, No. 2, 31.01.1994, p. 459-465.

Research output: Contribution to journalArticle

Kalapos, M. ; Cao, G. J. ; Kushner, S. R. ; Sarkar, N. / Identification of a Second Poly(A) Polymerase in Escherichia coli. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 198, No. 2. pp. 459-465.
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