Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain

V. Martella, M. Ciarlet, K. Bányai, E. Lorusso, A. Cavalli, M. Corrente, G. Elia, S. Arista, M. Camero, C. Desario, N. Decaro, A. Lavazza, C. Buonavoglia

Research output: Contribution to journalArticle

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Abstract

Rotavirus genome segment 4, encoding the spike outer capsid VP4 protein, of a porcine rotavirus (PoRV) strain, 134/04-15, identified in Italy was sequenced, and the predicted amino acid (aa) sequence was compared to those of all known VP4 (P) genotypes. The aa sequence of the full-length VP4 protein of the PoRV strain 134/04-15 showed aa identity values ranging from 59.7% (bovine strain KK3, P8[11]) to 86.09% (porcine strain A46, P[13]) with those of the remaining 25 P genotypes. Moreover, aa sequence analysis of the corresponding VP8* trypsin cleavage fragment revealed that the PoRV strain 134/04-15 shared low identity, ranging from 37.52% (bovine strain 993/83, P[17]) to 73.6% (porcine strain MDR-13, P[13]), with those of the remaining 25 P genotypes. Phylogenetic relationships showed that the VP4 of the PoRV strain 134/04-15 shares a common evolutionary origin with porcine P[13] and lapine P[22] rotavirus strains. Additional sequence analyses of the VP7, VP6, and NSP4 genes of the PoRV strain 134/04-15 revealed the highest VP7 aa identity (95.9%) to G5 porcine strains, a porcine-like VP6 within VP6 genogroup I, and a Wa-like (genotype B) NSP4, respectively. Altogether, these results indicate that the PoRV strain 134/04-15 should be considered as prototype of a new VP4 genotype, P[26], and provide further evidence for the vast genetic and antigenic diversity of group A rotaviruses.

Original languageEnglish
Pages (from-to)301-311
Number of pages11
JournalVirology
Volume346
Issue number2
DOIs
Publication statusPublished - Mar 15 2006

Fingerprint

Rotavirus
Swine
Genotype
Amino Acid Sequence
Serogroup
Amino Acids
Antigenic Variation
Protein Sequence Analysis
Capsid Proteins
Trypsin
Italy
Sequence Analysis
Genome

Keywords

  • Diarrhea
  • Genetic diversity
  • P genotype
  • Pigs
  • Rotavirus
  • VP4

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Martella, V., Ciarlet, M., Bányai, K., Lorusso, E., Cavalli, A., Corrente, M., ... Buonavoglia, C. (2006). Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. Virology, 346(2), 301-311. https://doi.org/10.1016/j.virol.2005.11.001

Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. / Martella, V.; Ciarlet, M.; Bányai, K.; Lorusso, E.; Cavalli, A.; Corrente, M.; Elia, G.; Arista, S.; Camero, M.; Desario, C.; Decaro, N.; Lavazza, A.; Buonavoglia, C.

In: Virology, Vol. 346, No. 2, 15.03.2006, p. 301-311.

Research output: Contribution to journalArticle

Martella, V, Ciarlet, M, Bányai, K, Lorusso, E, Cavalli, A, Corrente, M, Elia, G, Arista, S, Camero, M, Desario, C, Decaro, N, Lavazza, A & Buonavoglia, C 2006, 'Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain', Virology, vol. 346, no. 2, pp. 301-311. https://doi.org/10.1016/j.virol.2005.11.001
Martella, V. ; Ciarlet, M. ; Bányai, K. ; Lorusso, E. ; Cavalli, A. ; Corrente, M. ; Elia, G. ; Arista, S. ; Camero, M. ; Desario, C. ; Decaro, N. ; Lavazza, A. ; Buonavoglia, C. / Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. In: Virology. 2006 ; Vol. 346, No. 2. pp. 301-311.
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abstract = "Rotavirus genome segment 4, encoding the spike outer capsid VP4 protein, of a porcine rotavirus (PoRV) strain, 134/04-15, identified in Italy was sequenced, and the predicted amino acid (aa) sequence was compared to those of all known VP4 (P) genotypes. The aa sequence of the full-length VP4 protein of the PoRV strain 134/04-15 showed aa identity values ranging from 59.7{\%} (bovine strain KK3, P8[11]) to 86.09{\%} (porcine strain A46, P[13]) with those of the remaining 25 P genotypes. Moreover, aa sequence analysis of the corresponding VP8* trypsin cleavage fragment revealed that the PoRV strain 134/04-15 shared low identity, ranging from 37.52{\%} (bovine strain 993/83, P[17]) to 73.6{\%} (porcine strain MDR-13, P[13]), with those of the remaining 25 P genotypes. Phylogenetic relationships showed that the VP4 of the PoRV strain 134/04-15 shares a common evolutionary origin with porcine P[13] and lapine P[22] rotavirus strains. Additional sequence analyses of the VP7, VP6, and NSP4 genes of the PoRV strain 134/04-15 revealed the highest VP7 aa identity (95.9{\%}) to G5 porcine strains, a porcine-like VP6 within VP6 genogroup I, and a Wa-like (genotype B) NSP4, respectively. Altogether, these results indicate that the PoRV strain 134/04-15 should be considered as prototype of a new VP4 genotype, P[26], and provide further evidence for the vast genetic and antigenic diversity of group A rotaviruses.",
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