Identification of a novel cis-regulatory element for UV-B-induced transcription in Arabidopsis

Judit Safrany, Veronika Haasz, Zoltan Mate, Andrea Ciolfi, Balazs Feher, Attila Oravecz, Agnieszka Stec, Geza Dallmann, Giorgio Morelli, Roman Ulm, Ferenc Nagy

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38 Citations (Scopus)

Abstract

Ultraviolet-B light (UV-B) regulates the expression of genes in a wavelength- and fluence rate-dependent fashion. A signaling pathway consisting of CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) and UV RESISTANCE LOCUS 8 (UVR 8) mediates responsiveness to longer wavelength, low intensity UV-B light-activating, for example, HY5 gene expression. By contrast, transcription of another group of genes, including ANAC13, modulated by shorter wavelength, higher intensity UV-B is controlled by a yet unknown and largely COP1-independent signaling cascade. Here we provide evidence by promoter deletion analysis, and characterization of genetic mutants displaying aberrant expression patterns, that two cis-regulatory elements, designated MRE ANAC13 and UVBoxANAC13, are required for maximal UV-B induction of the ANAC13 gene in transgenic plants. These elements are located in the proximal 150-bp region of the ANAC13 promoter. They show no significant similarity to each other; the putative MREANAC13 (-AACCTT-) is closely related to MRECHS (-AACCTA-) found in the CHALCONE SYNTHASE (CHS) gene, whereas UVBoxANAC13 (with core sequence CAAG) represents a novel cis-regulatory element. The novel UVBoxANAC13 sequence is significantly enriched in the promoter region of a subset of UV-B-induced genes with similar activation properties as ANAC13. In addition, we demonstrate that expression of a chimeric gene containing only the dimerized 12-mer containing UVBoxANAC13 fused to a minimal CaMV35S promoter/luciferase reporter is (i) efficiently induced by shorter wavelength, higher intensity UV-B, but (ii) does not respond either to longer wavelength UV-B and red light or (iii) to abscisic acid treatment and osmotic, salt, heat and cold stresses.

Original languageEnglish
Pages (from-to)402-414
Number of pages13
JournalPlant Journal
Volume54
Issue number3
DOIs
Publication statusPublished - May 1 2008

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Keywords

  • ANAC13
  • Mutants
  • NAC family transcription factor
  • Promoter structure
  • Regulatory elements
  • Ultraviolet-B light

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Safrany, J., Haasz, V., Mate, Z., Ciolfi, A., Feher, B., Oravecz, A., Stec, A., Dallmann, G., Morelli, G., Ulm, R., & Nagy, F. (2008). Identification of a novel cis-regulatory element for UV-B-induced transcription in Arabidopsis. Plant Journal, 54(3), 402-414. https://doi.org/10.1111/j.1365-313X.2008.03435.x