Identification of a new, testis-specific sperm antigen localized on the principal piece of the spermatozoa tail in the fox (Vulpes vulpes)

Yann Verdier, Guillaume Farré, Nelly Rouet, Z. Kele, T. Janáky, Franck Boué

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Fox (Vulpes vulpes) sperm antigens were identified to assess them as a potential target for a contraceptive vaccine. We report here the cloning and sequencing of fSP13, a fox sperm protein of 97 kDa. The fSP13 protein was both auto- and iso-antigenic in foxes; it was recognized by sera of foxes immunized with fox sperm proteins and vasectomized foxes. The NH2-terminal sequence of fSP13 was determined, and a piece of cDNA was amplified from testicular RNA by reverse transcription polymerase chain reaction. This piece was used to screen a cDNA library from fox testis by Southern blot. A sequence of 1662 base pairs was obtained, including a major open reading frame coding for 498 amino acid. Mass spectrometry analysis confirmed the position of the open reading frame and the presence of posttranscriptional modifications. Analysis of the predicted amino acid sequence revealed no apparent transmembrane regions. Comparison of the protein sequence with the Prosite database demonstrated the presence of four potential N-linked glycosylation sites. The fSP13 bears the closest amino acid similarity to two human sperm proteins: fibrousheathin 2 and testis-specific calcium binding protein 86-VII. The deduced 80 N-terminal amino acid sequence also presents similarity with the RIIα domain. By using a serum against fSP13, this antigen was localized on the principal piece of the fox spermatozoa. Northern blot analysis showed that fSP13 is specifically expressed in testis. The fSP13 is one of the first fox sperm antigens to be cloned and sequenced.

Original languageEnglish
Pages (from-to)502-508
Number of pages7
JournalBiology of Reproduction
Volume72
Issue number2
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Spermatozoa
Tail
Testis
Antigens
Open Reading Frames
Proteins
Contraceptive Vaccines
Amino Acids
Calcium-Binding Proteins
Protein Sequence Analysis
Southern Blotting
Serum
Gene Library
Glycosylation
Base Pairing
Northern Blotting
Reverse Transcription
Organism Cloning
Amino Acid Sequence
Mass Spectrometry

Keywords

  • Fox
  • Gamete biology
  • Sperm
  • Testis

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Identification of a new, testis-specific sperm antigen localized on the principal piece of the spermatozoa tail in the fox (Vulpes vulpes). / Verdier, Yann; Farré, Guillaume; Rouet, Nelly; Kele, Z.; Janáky, T.; Boué, Franck.

In: Biology of Reproduction, Vol. 72, No. 2, 02.2005, p. 502-508.

Research output: Contribution to journalArticle

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