The presence of 1′-methylascorbigen, the most biologically active indole derivative of L-ascorbic acid, in broccoli has been proved by use of planar liquid and column liquid chromatography and mass spectrometry. Endogenous 1′-methylascorbigen was identified on the basis of its retention behavior, by means of the modified PRIMA method, and from its characteristic MALDI MS data. This paper is the first report of identification of 1′-methylascorbigen in the plant kingdom.
- High-performance liquid chromatography
- MALDI MS
- Overpressured-layer chromatography
- PRIMA method
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry