Identification of β-lactamases in human and bovine isolates of Staphylococcus aureus strains having borderline resistance to penicillinase-resistant penicillins (PRPs) with proteomic methods

Judit Szilvia Keseru, István Szabó, Zsuzsanna Gál, Orietta Massidda, Marina Mingoia, Éva Kaszanyitzky, Szilárd Jánosi, Julianna Hulvely, Attila Csorba, Krisztina Buzás, Éva Hunyadi-Gulyás, Katalin F. Medzihradszky, Sándor Biró

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Methicillin and oxacillin-hydrolyzing enzymes of 6 borderline methicillin-resistant and 1 methicillin-resistant Staphylococcus aureus strains isolated from human clinical samples and 4 borderline methicillin-resistant S. aureus strains isolated from bovine mastitis were investigated. As previous studies suggested the involvement of an additional enzyme besides the penicillinase BlaZ in the determination of borderline resistance, we analyzed the expressed extracellular and membrane-bound β-lactamases with 2-D gel electrophoresis and mass spectrometry. Our analysis showed that the penicillin-hydrolyzing BlaZ alone was responsible for the hydrolysis of both methicillin and oxacillin. All supernatant and membrane fractions contained the same enzyme with slight sequence variations. The size and pI of the proteins were also variable, probably due to spontaneous hydrolysis and/or posttranslational modifications. Interestingly, we found also cytotoxins and other virulence factors in some nitrocefin-hydrolyzing dots, suggesting that those proteins might have a role in the reduction of local antibiotic concentration.

Original languageEnglish
Pages (from-to)96-102
Number of pages7
JournalVeterinary Microbiology
Volume147
Issue number1-2
DOIs
Publication statusPublished - Jan 10 2011

Fingerprint

Penicillinase
beta-lactamase
methicillin
penicillins
Penicillins
Proteomics
proteomics
oxacillin
Staphylococcus aureus
Oxacillin
Methicillin
Methicillin-Resistant Staphylococcus aureus
cattle
Hydrolysis
Enzymes
enzymes
hydrolysis
Bovine Mastitis
cytotoxins
Methicillin Resistance

Keywords

  • Borderline methicillin-resistance
  • Membrane-bound β-lactamase
  • Penicillinase
  • Proteomics
  • PRPs-hydrolyzing enzyme
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Identification of β-lactamases in human and bovine isolates of Staphylococcus aureus strains having borderline resistance to penicillinase-resistant penicillins (PRPs) with proteomic methods. / Keseru, Judit Szilvia; Szabó, István; Gál, Zsuzsanna; Massidda, Orietta; Mingoia, Marina; Kaszanyitzky, Éva; Jánosi, Szilárd; Hulvely, Julianna; Csorba, Attila; Buzás, Krisztina; Hunyadi-Gulyás, Éva; Medzihradszky, Katalin F.; Biró, Sándor.

In: Veterinary Microbiology, Vol. 147, No. 1-2, 10.01.2011, p. 96-102.

Research output: Contribution to journalArticle

Keseru, Judit Szilvia ; Szabó, István ; Gál, Zsuzsanna ; Massidda, Orietta ; Mingoia, Marina ; Kaszanyitzky, Éva ; Jánosi, Szilárd ; Hulvely, Julianna ; Csorba, Attila ; Buzás, Krisztina ; Hunyadi-Gulyás, Éva ; Medzihradszky, Katalin F. ; Biró, Sándor. / Identification of β-lactamases in human and bovine isolates of Staphylococcus aureus strains having borderline resistance to penicillinase-resistant penicillins (PRPs) with proteomic methods. In: Veterinary Microbiology. 2011 ; Vol. 147, No. 1-2. pp. 96-102.
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