Identification and structural analysis of synthetic oligosaccharides of Shigella sonnei using MALDI-TOF MS

Gyöngyi Gyémánt, Anikó Tóth, István Bajza, Lili Kandra, András Lipták

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

MALDI-TOF mass spectroscopy was used for the molecular weight determination of protected synthetic oligosaccharides related to a cell surface bacterial polysaccharide. By-products containing chlorinated protecting groups caused isotopic patterns characteristic of the natural isotopic distribution of chlorine, were identified on the basis of isotopic distribution. 2,4,6-Trihydroxyacetophenone (THAP) as a matrix was better than 2,5-dihydroxybenzoic acid (DHB) for compounds containing chlorine, since monoisotopic resolution and no fragmentation were observed. In the post source decay (PSD) mode the identification of the oligosaccharide sequence through cleavage of the interglycosidic linkages was also possible, thus providing a sensitive and accurate tool for the structural verification of synthetic oligosaccharide intermediates.

Original languageEnglish
Pages (from-to)315-322
Number of pages8
JournalCarbohydrate Research
Volume334
Issue number4
DOIs
Publication statusPublished - Sep 7 2001

Keywords

  • MALDI-TOF MS
  • Oligosaccharide, Shigella sonnei
  • Post source decay (PSD)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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