Identification and quantification of lignans and sesquilignans in the fruits of Cnicus benedictus L. Quantitative chromatographic and spectroscopic approaches

Anna Sólyomváry, Gergo Tóth, Márta Kraszni, B. Noszál, I. Molnár-Perl, Imre Boldizsár

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Identification, quantification and isolation of lignans (Lig-s: arctiin, arctigenin and matairesinol) and sesquilignans (SLig-s: lappaol A, isolappaol A, lappaol C and isolappaol C), in Cnicus benedictus L. fruit (CBfr) were performed for the first time. Identity of Lig-s and SLig-s was confirmed by gas chromatography-mass spectrometry (GC-MS) and by high performance liquid chromatography-time-of-flight (HPLC-TOF) MS, while their detailed structures were defined by nuclear magnetic resonance (NMR) spectroscopy. As a novelty to the field, fruit part-specific accumulation of Lig-s and SLig-s under germination and their transformation during acidic and enzymatic hydrolyses were followed in a quantitative manner by HPLC-UV. It was shown that during germination, the spontaneous separation of Lig-s and SLig-s occurs: the fruit wall part accumulates SLig-s, while the embryo accumulates the Lig arctiin. It was confirmed that under optimized mild acidic conditions (50. °C, 2. M trifluoroacetic acid, TFA), lappaol C and isolappaol C can be transformed into lappaol A and isolappaol A, quantitatively. Analytical performance characteristics (reliability and reproducibility), in the HPLC-UV quantifications of Lig-s and SLig-s were defined by the relative standard deviation percentages (RSD%-s) of analyses (averages of RSD%-s. ≤. 3.7). Due to our new harmonized analysis system, CBfr proved to be a new and rich source of SLig-s: levels as high as 5.8. mmol/100. g were determined compared to the highest level (0.72. mmol/100. g) reported so far.

Original languageEnglish
Pages (from-to)238-246
Number of pages9
JournalMicrochemical Journal
Volume114
DOIs
Publication statusPublished - May 2014

Fingerprint

Lignans
Fruits
Trifluoroacetic Acid
Enzymatic hydrolysis
High performance liquid chromatography
Gas chromatography
Nuclear magnetic resonance spectroscopy
Mass spectrometry
arctiin

Keywords

  • Acidic/enzymatic hydrolyses
  • Fruit part-specific accumulation
  • GC-MS
  • Germination
  • HPLC-UV-TOF/MS
  • Quantification of lignans/sesquilignans

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Identification and quantification of lignans and sesquilignans in the fruits of Cnicus benedictus L. Quantitative chromatographic and spectroscopic approaches. / Sólyomváry, Anna; Tóth, Gergo; Kraszni, Márta; Noszál, B.; Molnár-Perl, I.; Boldizsár, Imre.

In: Microchemical Journal, Vol. 114, 05.2014, p. 238-246.

Research output: Contribution to journalArticle

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