Identification and measurement of endogenous formaldehyde in Datura innoxia Mill. callus tissues by oplc, HPLC and MALDI MS techniques

I. László, É Szoke, E. Tyihak, Zs Nemeth, L. Albert.

Research output: Contribution to journalArticle

Abstract

The purpose of the present study was to determine the presence of measurable amount of endogenous formaldehyde (HCHO) in callus tissue cultures of Datura innoxia Mill. applying different analytical methods. The effect of HCHO deprivation with dimedone - as abiotic stress - was also investigated on the growth and endogenous HCHO level in D. innoxia callus cultures. In our work, HCHO in dimedone adduct form (formaldemethone), has been isolated and characterised by overpressured layer chromatography (OPLC), high pressure liquid chromatography (HPLC), and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS). Endogenous HCHO deprivation with dimedone affected the cell proliferation in D. innoxia callus tissues as well.

Original languageEnglish
Pages (from-to)265-270
Number of pages6
JournalActa Horticulturae
Volume597
Publication statusPublished - Jan 30 2004

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Datura
matrix-assisted laser desorption-ionization mass spectrometry
formaldehyde
callus
high performance liquid chromatography
callus culture
abiotic stress
analytical methods
tissue culture
cell proliferation
chromatography
methodology
tissues

Keywords

  • Abiotic stress
  • Datura innoxia Mill.
  • Dimedone
  • Formaldemethone

ASJC Scopus subject areas

  • Horticulture

Cite this

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AU - Szoke, É

AU - Tyihak, E.

AU - Nemeth, Zs

AU - Albert., L.

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AB - The purpose of the present study was to determine the presence of measurable amount of endogenous formaldehyde (HCHO) in callus tissue cultures of Datura innoxia Mill. applying different analytical methods. The effect of HCHO deprivation with dimedone - as abiotic stress - was also investigated on the growth and endogenous HCHO level in D. innoxia callus cultures. In our work, HCHO in dimedone adduct form (formaldemethone), has been isolated and characterised by overpressured layer chromatography (OPLC), high pressure liquid chromatography (HPLC), and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS). Endogenous HCHO deprivation with dimedone affected the cell proliferation in D. innoxia callus tissues as well.

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