Identification and cloning of partial mbh2 gene cluster of hyperthermophile Aquifex pyrophilus

J. Lu, G. Rákhely, K. Kovács, C. Xiao, P. Zhou

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A 0.8 kb fragment of mbhS2 gene of Aquifex pyrophilus was obtained by PCR with designed primers basing mbhS2 gene of A. aeolicus. It showed 85% homology with the corresponding region of A. aeolicus. Using it as probe, a 5.0 kb Nco I fragment was fished out from the partial genomic library of A. pyrophilus. Then this fragment was cloned, subcloned and sequenced. The result revealed that the fragment contains the full length gene for the mbhS2, the gene orf1 and the first 366 bp of orf2. Comparison with mbhS2 and orf963 of A. aeolicus shows 81% and 60% homologies in amino acid sequence, respectively.

Original languageEnglish
Pages (from-to)674-679
Number of pages6
JournalWei sheng wu xue bao = Acta microbiologica Sinica
Volume41
Issue number6
Publication statusPublished - 2001

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Multigene Family
Organism Cloning
Genes
Amino Acid Sequence Homology
Genomic Library
Polymerase Chain Reaction

Cite this

Identification and cloning of partial mbh2 gene cluster of hyperthermophile Aquifex pyrophilus. / Lu, J.; Rákhely, G.; Kovács, K.; Xiao, C.; Zhou, P.

In: Wei sheng wu xue bao = Acta microbiologica Sinica, Vol. 41, No. 6, 2001, p. 674-679.

Research output: Contribution to journalArticle

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AU - Zhou, P.

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