Identification and characterization of two related murine genes, Eat2a and Eat2b, encoding single SH2-domain adapters

Silvia Calpe, Erika Erdos, Gongxian Liao, Ninghai Wang, Svend Rietdijk, Maria Simarro, Beata Scholtz, Jill Mooney, Hoon Lee Chang, Sun Shin Min, Éva Rajnavölgyi, John Schatzle, Herbert C. Morse, Cox Terhorst, Arpad Lanyi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Human EAT-2 (SH2D1B) and SLAM-associated protein (SAP) (SH2D1A) are single SH2-domain adapters, which bind to specific tyrosine residues in the cytoplasmic tail of six signaling lymphocytic activation molecule (SLAM) (SLAMF1)-related receptors. Here we report that, unlike in humans, the mouse and rat Eat2 genes are duplicated with an identical genomic organization. The coding regions of the mouse Eat2a and Eat2b genes share 91% identity at the nucleotide level and 84% at the protein level; similarly, segments of introns are highly conserved. Whereas expression of mouse Eat2a mRNA was detected in multiple tissues, Eat2b was only detectable in mouse natural killer cells, CD8+ T cells, and ovaries, suggesting a very restricted tissue expression of the latter. Both the EAT-2A and EAT-2B coimmunoprecipitated with mouse SLAM in transfected cells and augmented tyrosine phosphorylation of the cytoplasmic tail of SLAM. Both EAT-2A and EAT-2B bind to the Src-like kinases Fyn, Hck, Lyn, Lck, and Fgr, as determined by a yeast two-hybrid assay. However, unlike SAP, the EAT-2 proteins bind to their kinase domains and not to the SH3 domain of these kinases. Taken together, the data suggest that both EAT-2A and EAT-2B are adapters that recruit Src kinases to SLAM family receptors using a mechanism that is distinct from that of SAP.

Original languageEnglish
Pages (from-to)15-25
Number of pages11
JournalImmunogenetics
Volume58
Issue number1
DOIs
Publication statusPublished - Feb 1 2006

Keywords

  • Adapter proteins
  • EAT-2
  • SLAM
  • Src kinases

ASJC Scopus subject areas

  • Immunology
  • Genetics

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    Calpe, S., Erdos, E., Liao, G., Wang, N., Rietdijk, S., Simarro, M., Scholtz, B., Mooney, J., Chang, H. L., Min, S. S., Rajnavölgyi, É., Schatzle, J., Morse, H. C., Terhorst, C., & Lanyi, A. (2006). Identification and characterization of two related murine genes, Eat2a and Eat2b, encoding single SH2-domain adapters. Immunogenetics, 58(1), 15-25. https://doi.org/10.1007/s00251-005-0056-3