Identification and characterization of two mtf-1 genes in common carp

Ágnes Ferencz, E. Hermesz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Metal-responsive Transcription Factor (MTF-1) serves as an essential regulator of Zn2+ homeostasis via the activation of metallothionein gene expression. Only a single mtf-1 gene has been identified in any organism investigated previously. We report here the first evidence of the existence of two genes encoding MTF-1 proteins (mtf-1.1 and mtf-1.2). The expression patterns were followed in the liver, kidney, muscle, brain and heart by means of Northern hybridization and reverse transcription coupled polymerase chain reactions (RT-PCR). mtf-1.1 mRNA was detected in all tissues examined, with the highest level in the brain, and the lowest in the kidney and the liver. mtf-1.2 expression was detected exclusively in the brain. Cold shock and Cd2+ exposure influence the gene expression at the transcriptional level, in a stress-specific manner.

Original languageEnglish
Pages (from-to)238-243
Number of pages6
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume148
Issue number3
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Carps
Brain
Genes
Gene expression
Liver
Kidney
Gene Expression
Gene encoding
Metallothionein
Polymerase chain reaction
Transcription
Reverse Transcription
Muscle
Shock
Myocardium
Homeostasis
Metals
Chemical activation
Tissue
Polymerase Chain Reaction

Keywords

  • Brain
  • Cadmium
  • Cold shock
  • Isoforms
  • Metallothionein
  • MTF-1
  • Stress

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Identification and characterization of two mtf-1 genes in common carp. / Ferencz, Ágnes; Hermesz, E.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 148, No. 3, 09.2008, p. 238-243.

Research output: Contribution to journalArticle

@article{4c5c3870fd4e478abba5afc1305b8b69,
title = "Identification and characterization of two mtf-1 genes in common carp",
abstract = "The Metal-responsive Transcription Factor (MTF-1) serves as an essential regulator of Zn2+ homeostasis via the activation of metallothionein gene expression. Only a single mtf-1 gene has been identified in any organism investigated previously. We report here the first evidence of the existence of two genes encoding MTF-1 proteins (mtf-1.1 and mtf-1.2). The expression patterns were followed in the liver, kidney, muscle, brain and heart by means of Northern hybridization and reverse transcription coupled polymerase chain reactions (RT-PCR). mtf-1.1 mRNA was detected in all tissues examined, with the highest level in the brain, and the lowest in the kidney and the liver. mtf-1.2 expression was detected exclusively in the brain. Cold shock and Cd2+ exposure influence the gene expression at the transcriptional level, in a stress-specific manner.",
keywords = "Brain, Cadmium, Cold shock, Isoforms, Metallothionein, MTF-1, Stress",
author = "{\'A}gnes Ferencz and E. Hermesz",
year = "2008",
month = "9",
doi = "10.1016/j.cbpc.2008.06.001",
language = "English",
volume = "148",
pages = "238--243",
journal = "Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology",
issn = "0742-8413",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Identification and characterization of two mtf-1 genes in common carp

AU - Ferencz, Ágnes

AU - Hermesz, E.

PY - 2008/9

Y1 - 2008/9

N2 - The Metal-responsive Transcription Factor (MTF-1) serves as an essential regulator of Zn2+ homeostasis via the activation of metallothionein gene expression. Only a single mtf-1 gene has been identified in any organism investigated previously. We report here the first evidence of the existence of two genes encoding MTF-1 proteins (mtf-1.1 and mtf-1.2). The expression patterns were followed in the liver, kidney, muscle, brain and heart by means of Northern hybridization and reverse transcription coupled polymerase chain reactions (RT-PCR). mtf-1.1 mRNA was detected in all tissues examined, with the highest level in the brain, and the lowest in the kidney and the liver. mtf-1.2 expression was detected exclusively in the brain. Cold shock and Cd2+ exposure influence the gene expression at the transcriptional level, in a stress-specific manner.

AB - The Metal-responsive Transcription Factor (MTF-1) serves as an essential regulator of Zn2+ homeostasis via the activation of metallothionein gene expression. Only a single mtf-1 gene has been identified in any organism investigated previously. We report here the first evidence of the existence of two genes encoding MTF-1 proteins (mtf-1.1 and mtf-1.2). The expression patterns were followed in the liver, kidney, muscle, brain and heart by means of Northern hybridization and reverse transcription coupled polymerase chain reactions (RT-PCR). mtf-1.1 mRNA was detected in all tissues examined, with the highest level in the brain, and the lowest in the kidney and the liver. mtf-1.2 expression was detected exclusively in the brain. Cold shock and Cd2+ exposure influence the gene expression at the transcriptional level, in a stress-specific manner.

KW - Brain

KW - Cadmium

KW - Cold shock

KW - Isoforms

KW - Metallothionein

KW - MTF-1

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=49149117386&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49149117386&partnerID=8YFLogxK

U2 - 10.1016/j.cbpc.2008.06.001

DO - 10.1016/j.cbpc.2008.06.001

M3 - Article

C2 - 18602497

AN - SCOPUS:49149117386

VL - 148

SP - 238

EP - 243

JO - Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology

JF - Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology

SN - 0742-8413

IS - 3

ER -