Identification and characterization of T5-like bacteriophages representing two novel subgroups from food products

Domonkos Sváb, Linda Falgenhauer, Manfred Rohde, Judit Szabó, Trinad Chakraborty, I. Tóth

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During recent years, interest in the use of bacteriophages as biocontrol agents against foodborne pathogens has increased, particularly for members of the family Enterobacteriaceae, with pathogenic Escherichia coli, Shigella, and Salmonella strains among them. Here, we report the isolation and characterisation of 12 novel T5-like bacteriophages from confiscated food samples. All bacterophages effectively lysed E. coli K-12 strains and were able to infect pathogenic E. coli strains representing enterohaemorrhagic (EHEC), enteropathogenic (EPEC), enterotoxigenic (ETEC), and enteroinvasive (EIEC) pathotypes, Shigella dysenteriae, S. sonnei strains, as well as multidrug-resistant (MDR) E. coli and multiple strains representing different Salmonella enterica serovars. All the bacteriophages exhibited Siphoviridae morphology. Whole genome sequencing of the novel T5-like bacteriophages showed that they represent two distinct groups, with the genome-based grouping correlating to the different host spectra. As these bacteriophages are of food origin, their stability and lack of any virulence genes, as well as their broad and mutually complementary host spectrum makes these new T5-like bacteriophages valuable candidates for use as biocontrol agents against foodborne pathogenic enterobacteria.

Original languageEnglish
Article number202
JournalFrontiers in Microbiology
Volume9
Issue numberFEB
DOIs
Publication statusPublished - Feb 13 2018

Fingerprint

Bacteriophages
Food
Escherichia coli
Enterobacteriaceae
Siphoviridae
Shigella dysenteriae
Genome
Enteropathogenic Escherichia coli
Shigella
Salmonella enterica
Salmonella
Virulence
Genes

Keywords

  • Bacteriophages
  • Bio-control
  • Enterobacteriaceae
  • ESBL E. coli
  • Food safety
  • Phage genomics
  • T5-like phages

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Identification and characterization of T5-like bacteriophages representing two novel subgroups from food products. / Sváb, Domonkos; Falgenhauer, Linda; Rohde, Manfred; Szabó, Judit; Chakraborty, Trinad; Tóth, I.

In: Frontiers in Microbiology, Vol. 9, No. FEB, 202, 13.02.2018.

Research output: Contribution to journalArticle

Sváb, Domonkos ; Falgenhauer, Linda ; Rohde, Manfred ; Szabó, Judit ; Chakraborty, Trinad ; Tóth, I. / Identification and characterization of T5-like bacteriophages representing two novel subgroups from food products. In: Frontiers in Microbiology. 2018 ; Vol. 9, No. FEB.
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