Accurate sex identification of dioccious plants at the juvenile stage would be of great importance in the selection and breeding of these species. Marker-assisted selection - based on sex-associated molecular markers - provides a good way of achieving this goal. In order to identify sex-specific markers 21 decamer RAPD primers were tested on several dioecious hemp cultivars. Two of them (OPD05 and UBC354) generated male-specific markers. These two DNA fragments were isolated, cloned and sequenced. While the OPD05961 sequence revealed no differences when randomly chosen female and male genomic DNA was subjected to Southern analysis the UBC354151 probe resulted in differences in hybridisation signals. Both markers are unique, because there is no sequence with significant homology to the OPD05961 or UBC354 151 markers in databases. Male-specific RAPDs were converted to SCARs. These SCAR markers (SCAR323 and SCAR119) gave efficient identification of all male plants and were able to amplify a single DNA band in both cases. SCAR markers were used to analyse randomly chosen F 2 plants. The SCAR markers correlated to the sex of the segregating population, except for 2 male plants where these markers were missing.
|Translated title of the contribution||Identification and characterization of sex-specific molecular markers in hemp|
|Number of pages||17|
|Publication status||Published - Dec 2002|
ASJC Scopus subject areas
- Agronomy and Crop Science